Expression, Purification, and Characterization of Recombinant Human α1-Antitrypsin Produced Using Silkworm–Baculovirus Expression System

Yoshiki Morifuji, Jian Xu, Noriko Karasaki, Kazuhiro Iiyama, Daisuke Morokuma, Masato Hino, Akitsu Masuda, Takumi Yano, Hiroaki Mon, Takahiro Kusakabe, Man Lee

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

Human α1-antitrypsin (AAT) is the most abundant serine proteinase inhibitor (serpin) in the human plasma. Commercially available AAT for the medications of deficiency of α1-antitrypsin is mainly purified from human plasma. There is a high demand for a stable and low-cost supply of recombinant AAT (rAAT). In this study, the baculovirus expression vector system using silkworm larvae as host was employed and a large amount of highly active AAT was recovered from the silkworm serum (~ 15 mg/10 ml) with high purity. Both the enzymatic activity and stability of purified rAAT were comparable with those of commercial product. Our results provide an alternative method for mass production of the active rAAT in pharmaceutical use.

元の言語英語
ジャーナルMolecular Biotechnology
DOI
出版物ステータス受理済み/印刷中 - 1 1 2018

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Plasma (human)
Purification
Bombyx
Serine Proteinase Inhibitors
Drug products
Baculoviridae
Larva
Pharmaceutical Preparations
Costs
Costs and Cost Analysis
Serum
Peptide Hydrolases

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

これを引用

Expression, Purification, and Characterization of Recombinant Human α1-Antitrypsin Produced Using Silkworm–Baculovirus Expression System. / Morifuji, Yoshiki; Xu, Jian; Karasaki, Noriko; Iiyama, Kazuhiro; Morokuma, Daisuke; Hino, Masato; Masuda, Akitsu; Yano, Takumi; Mon, Hiroaki; Kusakabe, Takahiro; Lee, Man.

:: Molecular Biotechnology, 01.01.2018.

研究成果: ジャーナルへの寄稿記事

Morifuji, Yoshiki ; Xu, Jian ; Karasaki, Noriko ; Iiyama, Kazuhiro ; Morokuma, Daisuke ; Hino, Masato ; Masuda, Akitsu ; Yano, Takumi ; Mon, Hiroaki ; Kusakabe, Takahiro ; Lee, Man. / Expression, Purification, and Characterization of Recombinant Human α1-Antitrypsin Produced Using Silkworm–Baculovirus Expression System. :: Molecular Biotechnology. 2018.
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author = "Yoshiki Morifuji and Jian Xu and Noriko Karasaki and Kazuhiro Iiyama and Daisuke Morokuma and Masato Hino and Akitsu Masuda and Takumi Yano and Hiroaki Mon and Takahiro Kusakabe and Man Lee",
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AU - Morifuji, Yoshiki

AU - Xu, Jian

AU - Karasaki, Noriko

AU - Iiyama, Kazuhiro

AU - Morokuma, Daisuke

AU - Hino, Masato

AU - Masuda, Akitsu

AU - Yano, Takumi

AU - Mon, Hiroaki

AU - Kusakabe, Takahiro

AU - Lee, Man

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