TY - GEN
T1 - Extension and measurements on a phospholipid vesicle by use of dual-beam optical tweezers
AU - Ichikawa, Masatoshi
AU - Shitamichi, Yoko
AU - Kimura, Yasuyuki
PY - 2008/12/1
Y1 - 2008/12/1
N2 - A vesicle whose membrane is composed of a Phospholipid bilayer is called as a liposome. Since the fundamental structure of the membrane is the same as cell membranes, liposomes have been intensively studied as a simple model of live-cells. Here, we study the mechanical property of a micrometer-sized giant liposome through extending a single liposome from the inside by use of dual-beam optical tweezers. As the liposome is extended, the shape gradually changes from a sphere to a lemon-shape, and finally, a tubular membrane projection is exhibited. The surface tension and the bending rigidity of the lipid membrane are obtained from the measured force-extension curve. The present paper reviews both experimental and theoretical bases of this extension measurement method on the liposome with our experimental data.
AB - A vesicle whose membrane is composed of a Phospholipid bilayer is called as a liposome. Since the fundamental structure of the membrane is the same as cell membranes, liposomes have been intensively studied as a simple model of live-cells. Here, we study the mechanical property of a micrometer-sized giant liposome through extending a single liposome from the inside by use of dual-beam optical tweezers. As the liposome is extended, the shape gradually changes from a sphere to a lemon-shape, and finally, a tubular membrane projection is exhibited. The surface tension and the bending rigidity of the lipid membrane are obtained from the measured force-extension curve. The present paper reviews both experimental and theoretical bases of this extension measurement method on the liposome with our experimental data.
UR - http://www.scopus.com/inward/record.url?scp=62449098827&partnerID=8YFLogxK
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U2 - 10.1109/MHS.2008.4752425
DO - 10.1109/MHS.2008.4752425
M3 - Conference contribution
AN - SCOPUS:62449098827
SN - 9781424429196
T3 - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008
SP - 71
EP - 76
BT - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
T2 - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
Y2 - 6 November 2008 through 9 November 2008
ER -