TY - JOUR
T1 - Extension of the lifespan of caenorhabditis elegans by the use of electrolyzed reduced water
AU - Yan, Hanxu
AU - Tian, Huaize
AU - Kinjo, Tomoya
AU - Hamasaki, Takeki
AU - Tomimatsu, Kosuke
AU - Nakamichi, Noboru
AU - Teruya, Kiichiro
AU - Kabayama, Shigeru
AU - Shirahata, Sanetaka
PY - 2010
Y1 - 2010
N2 - Electrolyzed reduced water (ERW) has attracted much attention because of its therapeutic effects. In the present study, a new culture medium, which we designated Water medium, was developed to elucidate the effects of ERW on the lifespan of Caenorhabditis elegans. Wild-type C. elegans had a significantly shorter lifespan in Water medium than in conventional S medium. However, worms cultured in ERW-Water medium exhibited a significantly extended lifespan (from 11% to 41%) compared with worms cultured in ultrapure water-Water medium. There was no difference between the lifespans of worms cultured in ERW-S medium and ultrapure water-S medium. Nematodes cultured in ultrapure water-Water medium showed significantly higher levels of reactive oxygen species than those cultured in ultrapure water-S medium. Moreover, ERW-Water medium significantly reduced the ROS accumulation induced in the worms by paraquat, suggesting that ERW-Water medium extends the longevity of nematodes at least partly by scavenging ROS.
AB - Electrolyzed reduced water (ERW) has attracted much attention because of its therapeutic effects. In the present study, a new culture medium, which we designated Water medium, was developed to elucidate the effects of ERW on the lifespan of Caenorhabditis elegans. Wild-type C. elegans had a significantly shorter lifespan in Water medium than in conventional S medium. However, worms cultured in ERW-Water medium exhibited a significantly extended lifespan (from 11% to 41%) compared with worms cultured in ultrapure water-Water medium. There was no difference between the lifespans of worms cultured in ERW-S medium and ultrapure water-S medium. Nematodes cultured in ultrapure water-Water medium showed significantly higher levels of reactive oxygen species than those cultured in ultrapure water-S medium. Moreover, ERW-Water medium significantly reduced the ROS accumulation induced in the worms by paraquat, suggesting that ERW-Water medium extends the longevity of nematodes at least partly by scavenging ROS.
UR - http://www.scopus.com/inward/record.url?scp=78049387133&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78049387133&partnerID=8YFLogxK
U2 - 10.1271/bbb.100250
DO - 10.1271/bbb.100250
M3 - Article
C2 - 20944427
AN - SCOPUS:78049387133
VL - 74
SP - 2011
EP - 2015
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 10
ER -