Extracellular polysaccharide-protein complexes of a harmful alga mediate the allelopathic control it exerts within the phytoplankton community

Yasuhiro Yamasaki, Tomoyuki Shikata, Atsushi Nukata, Satoko Ichiki, Sou Nagasoe, Tadashi Matsubara, Yohei Shimasaki, Miki Nakao, Kenichi Yamaguchi, Yuji Oshima, Tatsuya Oda, Makoto Ito, Ian R. Jenkinson, Makio Asakawa, Tsuneo Honjo

研究成果: ジャーナルへの寄稿記事

39 引用 (Scopus)

抄録

The goal of this study was to examine the significance of allelopathy by the raphidophyte Heterosigma akashiwo in a multispecies phytoplankton community in the field. Towards this aim, we sought allelochemicals of H. akashiwo, which had allelopathic effect both in laboratory experiments and in the field. As an initial step, we showed that the allelopathic effects of H. akashiwo filtrate were both species-specific and dependent upon the cell density of the target species. Secondly, we found for the first time that extracellular, high-molecular weight allelochemicals that is, polysaccharide-protein complexes (APPCs) were produced by a marine phytoplankton species, H. akashiwo. Thirdly, we indicated that the purified APPCs selectively inhibited the growth of the diatom Skeletonema costatum that is a major competitor of H. akashiwo, and thereby tended to promote the formation of monospecific H. akashiwo blooms. Furthermore, we demonstrated that the inhibitory effect of APPCs on the growth of the diatoms was determined by binding to the cell surface of the target species. Finally, we succeeded in the detection of APPCs in the field samples at concentrations exceeding their experimentally determined action threshold during the H. akashiwo bloom. Strategies for ecosystem control, including mitigation of harmful algal blooms (HABs), should take into account that red-tide organisms like H. akashiwo are already part of complex webs involving inter-specific allelopathic inhibition and ecosystem control during their dense blooms.

元の言語英語
ページ(範囲)808-817
ページ数10
ジャーナルISME Journal
3
発行部数7
DOI
出版物ステータス出版済み - 7 1 2009

Fingerprint

algae
Phytoplankton
allelochemical
Pheromones
allelochemicals
polysaccharide
Polysaccharides
polysaccharides
phytoplankton
alga
algal bloom
Harmful Algal Bloom
protein
Diatoms
Bacillariophyceae
Proteins
proteins
algal blooms
Ecosystem
Allelopathy

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

これを引用

Extracellular polysaccharide-protein complexes of a harmful alga mediate the allelopathic control it exerts within the phytoplankton community. / Yamasaki, Yasuhiro; Shikata, Tomoyuki; Nukata, Atsushi; Ichiki, Satoko; Nagasoe, Sou; Matsubara, Tadashi; Shimasaki, Yohei; Nakao, Miki; Yamaguchi, Kenichi; Oshima, Yuji; Oda, Tatsuya; Ito, Makoto; Jenkinson, Ian R.; Asakawa, Makio; Honjo, Tsuneo.

:: ISME Journal, 巻 3, 番号 7, 01.07.2009, p. 808-817.

研究成果: ジャーナルへの寄稿記事

Yamasaki, Y, Shikata, T, Nukata, A, Ichiki, S, Nagasoe, S, Matsubara, T, Shimasaki, Y, Nakao, M, Yamaguchi, K, Oshima, Y, Oda, T, Ito, M, Jenkinson, IR, Asakawa, M & Honjo, T 2009, 'Extracellular polysaccharide-protein complexes of a harmful alga mediate the allelopathic control it exerts within the phytoplankton community', ISME Journal, 巻. 3, 番号 7, pp. 808-817. https://doi.org/10.1038/ismej.2009.24
Yamasaki, Yasuhiro ; Shikata, Tomoyuki ; Nukata, Atsushi ; Ichiki, Satoko ; Nagasoe, Sou ; Matsubara, Tadashi ; Shimasaki, Yohei ; Nakao, Miki ; Yamaguchi, Kenichi ; Oshima, Yuji ; Oda, Tatsuya ; Ito, Makoto ; Jenkinson, Ian R. ; Asakawa, Makio ; Honjo, Tsuneo. / Extracellular polysaccharide-protein complexes of a harmful alga mediate the allelopathic control it exerts within the phytoplankton community. :: ISME Journal. 2009 ; 巻 3, 番号 7. pp. 808-817.
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abstract = "The goal of this study was to examine the significance of allelopathy by the raphidophyte Heterosigma akashiwo in a multispecies phytoplankton community in the field. Towards this aim, we sought allelochemicals of H. akashiwo, which had allelopathic effect both in laboratory experiments and in the field. As an initial step, we showed that the allelopathic effects of H. akashiwo filtrate were both species-specific and dependent upon the cell density of the target species. Secondly, we found for the first time that extracellular, high-molecular weight allelochemicals that is, polysaccharide-protein complexes (APPCs) were produced by a marine phytoplankton species, H. akashiwo. Thirdly, we indicated that the purified APPCs selectively inhibited the growth of the diatom Skeletonema costatum that is a major competitor of H. akashiwo, and thereby tended to promote the formation of monospecific H. akashiwo blooms. Furthermore, we demonstrated that the inhibitory effect of APPCs on the growth of the diatoms was determined by binding to the cell surface of the target species. Finally, we succeeded in the detection of APPCs in the field samples at concentrations exceeding their experimentally determined action threshold during the H. akashiwo bloom. Strategies for ecosystem control, including mitigation of harmful algal blooms (HABs), should take into account that red-tide organisms like H. akashiwo are already part of complex webs involving inter-specific allelopathic inhibition and ecosystem control during their dense blooms.",
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AU - Yamasaki, Yasuhiro

AU - Shikata, Tomoyuki

AU - Nukata, Atsushi

AU - Ichiki, Satoko

AU - Nagasoe, Sou

AU - Matsubara, Tadashi

AU - Shimasaki, Yohei

AU - Nakao, Miki

AU - Yamaguchi, Kenichi

AU - Oshima, Yuji

AU - Oda, Tatsuya

AU - Ito, Makoto

AU - Jenkinson, Ian R.

AU - Asakawa, Makio

AU - Honjo, Tsuneo

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