Extraction of cell-free DNA from urine, using polylysine-coated silica particles

Sho Takano, Qingjiang Hu, Takaki Amamoto, Paulo Refinetti, Koshi Mimori, Takashi Funatsu, Masaru Kato

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

DNA analysis is used for a variety of purposes, including disease diagnosis and DNA profiling; this involves extracting DNA from living organisms. In this study, we prepared polycationic silica particles to extract DNA that has the negatively charged phosphate backbone from solution. The coated particles were prepared by mixing conventional silica gel particles and poly-Lys; these particles could efficiently extract 1.3 μg of cell-free DNA from 50 mL of (male) urine. It is expected that these easily prepared particles (just a mixture of two commercially available chemicals) can be used as a noninvasive diagnostic tool for genetic disorders such as cancer, diabetes, and hypertension. [Figure not available: see fulltext.].

元の言語英語
ページ(範囲)4021-4025
ページ数5
ジャーナルAnalytical and Bioanalytical Chemistry
409
発行部数16
DOI
出版物ステータス出版済み - 6 1 2017

Fingerprint

Polylysine
Silicon Dioxide
Urine
DNA
Inborn Genetic Diseases
DNA Fingerprinting
Silica Gel
Cell Extracts
Medical problems
Phosphates
Hypertension
Neoplasms

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry

これを引用

Extraction of cell-free DNA from urine, using polylysine-coated silica particles. / Takano, Sho; Hu, Qingjiang; Amamoto, Takaki; Refinetti, Paulo; Mimori, Koshi; Funatsu, Takashi; Kato, Masaru.

:: Analytical and Bioanalytical Chemistry, 巻 409, 番号 16, 01.06.2017, p. 4021-4025.

研究成果: ジャーナルへの寄稿記事

Takano, Sho ; Hu, Qingjiang ; Amamoto, Takaki ; Refinetti, Paulo ; Mimori, Koshi ; Funatsu, Takashi ; Kato, Masaru. / Extraction of cell-free DNA from urine, using polylysine-coated silica particles. :: Analytical and Bioanalytical Chemistry. 2017 ; 巻 409, 番号 16. pp. 4021-4025.
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