Fabrication and histological evaluation of porous carbonate apatite block from gypsum block containing spherical phenol resin as a porogen

The utility of carbonate apatite (CO₃Ap) as a bone substitute has been demonstrated. The feasibility of fabricating macroporous CO₃Ap was evaluated through a two-step dissolution-precipitation reaction using gypsum as the precursor and spherical phenol resin as the porogen. Porogen-containing gypsum was heated to burn out the porogen and to fabricate macroporous structures. Gypsum transformed into CaCO₃ upon immersion in a sodium carbonate solution, while maintaining its macroporous structure. Next, CaCO₃ transformed into CO₃Ap upon immersion in a Na₂HPO₄ solution while maintaining its macroporous structure. The utility of the macroporous CO₃Ap for histologically reconstructing bone defects was evaluated in rabbit femurs. After 4 weeks, a much larger bone was formed inside the macroporous CO₃Ap than that inside non-macroporous CO₃Ap and macroporous hydroxyapatite (HAp). A larger amount of bone was observed inside non-macroporous CO₃Ap than inside macroporous HAp. The bone defects were completely reconstructed within 12 weeks using macroporous CO₃Ap. In conclusion, macroporous CO₃Ap has good potential as an ideal bone substitute.