Fabrication of 3D tissue-like structure using magnetite nanoparticles and magnetic force

Akira Ito, Kousuke Ino, Kazunori Shimizu, Hiroyuki Honda, Masamichi Kamihira

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

3 被引用数 (Scopus)

抄録

Novel technologies to establish 3D tissue-like constructs are desired for tissue engineering. In the present study, magnetic force and magnetite nanoparticles were used to construct a layered mesenchymal stem cell (MSC) sheet, a layered cardiomyocyte sheet, and a layered fibroblast sheet involving capillaries. Magnetite cationic liposomes (MCLs) were taken up by the target cells. When a magnet was set under a tissue culture dish, magnetically labeled target cells were attracted and then adhered to form a layered cell sheet. MSC sheets were harvested and transplanted into the bone defect in cranium of nude rats, resulted in formation of new bones surrounded by osteoblast-like cells. For cardiomyocyte sheets, the immunofluorescence staining revealed the existence of gap junctions within the cardiomyocyte sheets. For fibroblast sheets, normal human dermal fibroblasts (NHDFs) sheets contained the major dermal extracellular matrix components. Human umbilical vein endothelial cells (HUVECs) were co-cultured with NIIDF sheets, resulted in tube-like formation of HUVECs. These results suggest that this novel use of magnetite nanoparticles and magnetic force, which we refer to as "magnetic force-based tissue engineering (Mag-TE)", offers a major advancement in tissue engineering.

本文言語英語
ホスト出版物のタイトル2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
DOI
出版ステータス出版済み - 12 1 2006
イベント2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS - Nagoya, 日本
継続期間: 11 5 200611 8 2006

出版物シリーズ

名前2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS

その他

その他2006 IEEE International Symposium on Micro-Nano Mechanical and Human Science, MHS
国/地域日本
CityNagoya
Period11/5/0611/8/06

All Science Journal Classification (ASJC) codes

  • 計算力学
  • 制御およびシステム工学
  • 人的要因と人間工学

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