TY - CHAP
T1 - Fabrication of elasticity-tunable gelatinous gel for mesenchymal stem cell culture
AU - Kuboki, Thasaneeya
AU - Kidoaki, Satoru
PY - 2016
Y1 - 2016
N2 - Surface elasticity or stiffness of an underlying substrate may regulate cellular functions such as adhesion, proliferation, signaling, differentiation, and migration. Recent studies have reported on the development of biomaterials to control stem cell fate determination via the stiffness of the culture substrates. In this chapter, we provide a detailed protocol for fabricating elasticity-tunable gelatinous hydrogels for stem cell culture with photo-induced or thermo-induced crosslinking of well-developed styrenated gelatin (StG). We also include the detailed application of gelatinous gel for mesenchymal stem cell (MSC) culture and sample collection for transcriptional and proteomic analysis.
AB - Surface elasticity or stiffness of an underlying substrate may regulate cellular functions such as adhesion, proliferation, signaling, differentiation, and migration. Recent studies have reported on the development of biomaterials to control stem cell fate determination via the stiffness of the culture substrates. In this chapter, we provide a detailed protocol for fabricating elasticity-tunable gelatinous hydrogels for stem cell culture with photo-induced or thermo-induced crosslinking of well-developed styrenated gelatin (StG). We also include the detailed application of gelatinous gel for mesenchymal stem cell (MSC) culture and sample collection for transcriptional and proteomic analysis.
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U2 - 10.1007/978-1-4939-3584-0_25
DO - 10.1007/978-1-4939-3584-0_25
M3 - Chapter
C2 - 27236687
AN - SCOPUS:84990187837
T3 - Methods in Molecular Biology
SP - 425
EP - 441
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -