Fabrication of elasticity-tunable gelatinous gel for mesenchymal stem cell culture

研究成果: 著書/レポートタイプへの貢献

抄録

Surface elasticity or stiffness of an underlying substrate may regulate cellular functions such as adhesion, proliferation, signaling, differentiation, and migration. Recent studies have reported on the development of biomaterials to control stem cell fate determination via the stiffness of the culture substrates. In this chapter, we provide a detailed protocol for fabricating elasticity-tunable gelatinous hydrogels for stem cell culture with photo-induced or thermo-induced crosslinking of well-developed styrenated gelatin (StG). We also include the detailed application of gelatinous gel for mesenchymal stem cell (MSC) culture and sample collection for transcriptional and proteomic analysis.

元の言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版者Humana Press Inc.
ページ425-441
ページ数17
DOI
出版物ステータス出版済み - 1 1 2016

出版物シリーズ

名前Methods in Molecular Biology
1416
ISSN(印刷物)1064-3745

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Elasticity
Mesenchymal Stromal Cells
Stem Cells
Cell Culture Techniques
Gels
Hydrogels
Biocompatible Materials
Gelatin
Proteomics

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

これを引用

Kuboki, T., & Kidoaki, S. (2016). Fabrication of elasticity-tunable gelatinous gel for mesenchymal stem cell culture. : Methods in Molecular Biology (pp. 425-441). (Methods in Molecular Biology; 巻数 1416). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3584-0_25

Fabrication of elasticity-tunable gelatinous gel for mesenchymal stem cell culture. / Kuboki, Thasaneeya; Kidoaki, Satoru.

Methods in Molecular Biology. Humana Press Inc., 2016. p. 425-441 (Methods in Molecular Biology; 巻 1416).

研究成果: 著書/レポートタイプへの貢献

Kuboki, T & Kidoaki, S 2016, Fabrication of elasticity-tunable gelatinous gel for mesenchymal stem cell culture. : Methods in Molecular Biology. Methods in Molecular Biology, 巻. 1416, Humana Press Inc., pp. 425-441. https://doi.org/10.1007/978-1-4939-3584-0_25
Kuboki T, Kidoaki S. Fabrication of elasticity-tunable gelatinous gel for mesenchymal stem cell culture. : Methods in Molecular Biology. Humana Press Inc. 2016. p. 425-441. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-3584-0_25
Kuboki, Thasaneeya ; Kidoaki, Satoru. / Fabrication of elasticity-tunable gelatinous gel for mesenchymal stem cell culture. Methods in Molecular Biology. Humana Press Inc., 2016. pp. 425-441 (Methods in Molecular Biology).
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