Fabrication of elasticity-tunable gelatinous gel for mesenchymal stem cell culture

研究成果: 書籍/レポート タイプへの寄稿

1 被引用数 (Scopus)

抄録

Surface elasticity or stiffness of an underlying substrate may regulate cellular functions such as adhesion, proliferation, signaling, differentiation, and migration. Recent studies have reported on the development of biomaterials to control stem cell fate determination via the stiffness of the culture substrates. In this chapter, we provide a detailed protocol for fabricating elasticity-tunable gelatinous hydrogels for stem cell culture with photo-induced or thermo-induced crosslinking of well-developed styrenated gelatin (StG). We also include the detailed application of gelatinous gel for mesenchymal stem cell (MSC) culture and sample collection for transcriptional and proteomic analysis.

本文言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ425-441
ページ数17
DOI
出版ステータス出版済み - 2016

出版物シリーズ

名前Methods in Molecular Biology
1416
ISSN(印刷版)1064-3745

!!!All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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