抄録
Surface elasticity or stiffness of an underlying substrate may regulate cellular functions such as adhesion, proliferation, signaling, differentiation, and migration. Recent studies have reported on the development of biomaterials to control stem cell fate determination via the stiffness of the culture substrates. In this chapter, we provide a detailed protocol for fabricating elasticity-tunable gelatinous hydrogels for stem cell culture with photo-induced or thermo-induced crosslinking of well-developed styrenated gelatin (StG). We also include the detailed application of gelatinous gel for mesenchymal stem cell (MSC) culture and sample collection for transcriptional and proteomic analysis.
| 元の言語 | 英語 |
|---|---|
| ホスト出版物のタイトル | Methods in Molecular Biology |
| 出版者 | Humana Press Inc. |
| ページ | 425-441 |
| ページ数 | 17 |
| DOI | |
| 出版物ステータス | 出版済み - 1 1 2016 |
出版物シリーズ
| 名前 | Methods in Molecular Biology |
|---|---|
| 巻 | 1416 |
| ISSN（印刷物） | 1064-3745 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
これを引用
Fabrication of elasticity-tunable gelatinous gel for mesenchymal stem cell culture. / Kuboki, Thasaneeya; Kidoaki, Satoru.
Methods in Molecular Biology. Humana Press Inc., 2016. p. 425-441 (Methods in Molecular Biology; 巻 1416).研究成果: 著書/レポートタイプへの貢献 › 章
}
TY - CHAP
T1 - Fabrication of elasticity-tunable gelatinous gel for mesenchymal stem cell culture
AU - Kuboki, Thasaneeya
AU - Kidoaki, Satoru
PY - 2016/1/1
Y1 - 2016/1/1
N2 - Surface elasticity or stiffness of an underlying substrate may regulate cellular functions such as adhesion, proliferation, signaling, differentiation, and migration. Recent studies have reported on the development of biomaterials to control stem cell fate determination via the stiffness of the culture substrates. In this chapter, we provide a detailed protocol for fabricating elasticity-tunable gelatinous hydrogels for stem cell culture with photo-induced or thermo-induced crosslinking of well-developed styrenated gelatin (StG). We also include the detailed application of gelatinous gel for mesenchymal stem cell (MSC) culture and sample collection for transcriptional and proteomic analysis.
AB - Surface elasticity or stiffness of an underlying substrate may regulate cellular functions such as adhesion, proliferation, signaling, differentiation, and migration. Recent studies have reported on the development of biomaterials to control stem cell fate determination via the stiffness of the culture substrates. In this chapter, we provide a detailed protocol for fabricating elasticity-tunable gelatinous hydrogels for stem cell culture with photo-induced or thermo-induced crosslinking of well-developed styrenated gelatin (StG). We also include the detailed application of gelatinous gel for mesenchymal stem cell (MSC) culture and sample collection for transcriptional and proteomic analysis.
UR - http://www.scopus.com/inward/record.url?scp=84990187837&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84990187837&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-3584-0_25
DO - 10.1007/978-1-4939-3584-0_25
M3 - Chapter
C2 - 27236687
AN - SCOPUS:84990187837
T3 - Methods in Molecular Biology
SP - 425
EP - 441
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -