Facilitation of proteasomal degradation of p27Kip1 by N-terminal cleavage and their sequence requirements

Katsuya Hirano, Eikichi Ihara, Mayumi Hirano, Junji Nishimura, Hajime Nawata, Hideo Kanaide

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

The sequence requirement for N-terminal cleavage and the proteasomal degradation of p27Kip1 and their relationship was investigated. Residues 5-8 were required for the cleavage and the mutation of S10 to E inhibited the cleavage. The C-terminal PEST sequence was necessary for the degradation and residue R165 was found to play an important role in the degradation. The inhibition of the cleavage by deleting residues 5-8 inhibited the degradation, while the fragment mimicking the cleavage product accelerated the degradation. Both the cleavage and degradation demonstrated a similar sensitivity toward proteasome inhibitors and ATP depletion. These two processes are thus suggested to be tightly linked and sequential.

本文言語英語
ページ(範囲)111-115
ページ数5
ジャーナルFEBS Letters
574
1-3
DOI
出版ステータス出版済み - 9 10 2004

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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