Facilitation of proteasomal degradation of p27Kip1 by N-terminal cleavage and their sequence requirements

Katsuya Hirano, Eikichi Ihara, Mayumi Hirano, Junji Nishimura, Hajime Nawata, Hideo Kanaide

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

The sequence requirement for N-terminal cleavage and the proteasomal degradation of p27Kip1 and their relationship was investigated. Residues 5-8 were required for the cleavage and the mutation of S10 to E inhibited the cleavage. The C-terminal PEST sequence was necessary for the degradation and residue R165 was found to play an important role in the degradation. The inhibition of the cleavage by deleting residues 5-8 inhibited the degradation, while the fragment mimicking the cleavage product accelerated the degradation. Both the cleavage and degradation demonstrated a similar sensitivity toward proteasome inhibitors and ATP depletion. These two processes are thus suggested to be tightly linked and sequential.

元の言語英語
ページ(範囲)111-115
ページ数5
ジャーナルFEBS Letters
574
発行部数1-3
DOI
出版物ステータス出版済み - 9 10 2004

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S 10
Proteasome Inhibitors
Adenosine Triphosphate
Degradation
Mutation

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

これを引用

Facilitation of proteasomal degradation of p27Kip1 by N-terminal cleavage and their sequence requirements. / Hirano, Katsuya; Ihara, Eikichi; Hirano, Mayumi; Nishimura, Junji; Nawata, Hajime; Kanaide, Hideo.

:: FEBS Letters, 巻 574, 番号 1-3, 10.09.2004, p. 111-115.

研究成果: ジャーナルへの寄稿記事

Hirano, Katsuya ; Ihara, Eikichi ; Hirano, Mayumi ; Nishimura, Junji ; Nawata, Hajime ; Kanaide, Hideo. / Facilitation of proteasomal degradation of p27Kip1 by N-terminal cleavage and their sequence requirements. :: FEBS Letters. 2004 ; 巻 574, 番号 1-3. pp. 111-115.
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