TY - JOUR
T1 - Fate redirection of hippocampal astrocytes toward neuronal lineage by aggregate culture
AU - Yanagisawa, Makoto
AU - Nakashima, Kinichi
AU - Ochiai, Wataru
AU - Takizawa, Takumi
AU - Setoguchi, Takao
AU - Uemura, Atsumi
AU - Takizawa, Makiko
AU - Nobuhisa, Ikuo
AU - Taga, Tetsuya
N1 - Funding Information:
We thank Yamanouchi Pharmaceutical for BMP2, Ms. Y. Noguchi for her secretarial assistance and Ms. K. Kaneko for her technical help. This work was supported by a Grant-in-Aid from Ministry of Education, Culture, Sports, Science and Technology, Human Frontier Science Program, Ichiro Kanehara Foundation, Inamori Foundation, Kato Memorial Bioscience Foundation and Naito Foundation. M.Y. was supported by the Research Fellowships of the Japan Society for Promotion of Science for Young Scientists.
PY - 2005/10
Y1 - 2005/10
N2 - Mammalian cells that have been committed to a certain cell lineage cannot be directed to other lineages. However, some astrocytes in the mammalian brains have been reported to represent plasticity to redirect to other cell lineages. We found that mouse hippocampal astrocytes cultured in aggregate forms of "astrosphere", redirected to MAP2-positive immature neurons. In astrospheres, basic HLH factors positively regulating neuronal differentiation were up-regulated and Id3 inhibiting basic HLH factors was down-regulated. Ectopic Id3 induction repressed redirection of astrocytes to a neuronal lineage, suggesting that astrosphere formation induced plasticity of astrocytes by changing the gene expression patterns.
AB - Mammalian cells that have been committed to a certain cell lineage cannot be directed to other lineages. However, some astrocytes in the mammalian brains have been reported to represent plasticity to redirect to other cell lineages. We found that mouse hippocampal astrocytes cultured in aggregate forms of "astrosphere", redirected to MAP2-positive immature neurons. In astrospheres, basic HLH factors positively regulating neuronal differentiation were up-regulated and Id3 inhibiting basic HLH factors was down-regulated. Ectopic Id3 induction repressed redirection of astrocytes to a neuronal lineage, suggesting that astrosphere formation induced plasticity of astrocytes by changing the gene expression patterns.
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U2 - 10.1016/j.neures.2005.06.015
DO - 10.1016/j.neures.2005.06.015
M3 - Article
C2 - 16112216
AN - SCOPUS:24044435529
VL - 53
SP - 176
EP - 182
JO - Neuroscience Research
JF - Neuroscience Research
SN - 0168-0102
IS - 2
ER -