Fat/vessel-derived secretory protein (Favine)/CCDC3 is involved in lipid accumulation

Sachiko Kobayashi, Atsunori Fukuhara, Michio Otsuki, Takayoshi Suganami, Yoshihiro Ogawa, Eiichi Morii, Iichiro Shimomura

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

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We previously identified a novel gene encoding Favine/CCDC3 (NCBI protein entry NP-083080), a possible secretory factor, the mRNA of which is highly expressed in adipose tissue and the aorta. The Favine mRNA levels are increased in the course of differentiation of rat primary adipocytes and are more elevated in the adipose tissue of genetically obese and diet-induced obese mice than in lean mice. However, its biological function has not yet been elucidated until now. Here, we tested the hypothesis that Favine is involved in lipid metabolism in adipocytes. We found that overexpression of Favine promoted 3T3-L1 adipocyte differentiation. To further investigate the function of Favine in vivo, we generated Favine knock-out (KO) mice. Favine KO mice exhibited a lean phenotype as they aged. The weights of white adipose tissue and liver were less, and adipocyte size was smaller in Favine KO mice compared with wild-type littermates (WT). Expression levels of lipogenic genes, such as fatty-acid synthase (FAS), acetyl-CoA carboxylase α (ACC1), and diacylglycerol O-acyltransferase-2 (Dgat2), were decreased in adipose tissue of Favine KO mice. In 1-year-old mice, Favine deficiency decreased the number of inflammatory cells in white adipose tissue and diminished hepatic steatosis. In vitro, deficiency of Favine attenuated differentiation of primary adipocytes. Taken together, these data demonstrate that Favine has adipogenic and lipogenic effects on adipocytes.

元の言語英語
ページ(範囲)7443-7451
ページ数9
ジャーナルJournal of Biological Chemistry
290
発行部数12
DOI
出版物ステータス出版済み - 3 20 2015
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Kobayashi, S., Fukuhara, A., Otsuki, M., Suganami, T., Ogawa, Y., Morii, E., & Shimomura, I. (2015). Fat/vessel-derived secretory protein (Favine)/CCDC3 is involved in lipid accumulation. Journal of Biological Chemistry, 290(12), 7443-7451. https://doi.org/10.1074/jbc.M114.592493