FcεIγ-ITAM Is Differentially Required for Mast Cell Function in Vivo

Daiju Sakurai, Sho Yamasaki, Kanako Arase, Seung Yong Park, Hisashi Arase, Akiyoshi Konno, Takashi Saito

    研究成果: ジャーナルへの寄稿学術誌査読

    41 被引用数 (Scopus)

    抄録

    The cross-linking of IgE-bound FcεRI by Ags triggers mast cell activation leading to allergic reactions. The in vivo contribution of FcγRIγ signaling to IgE/FcεRI-mediated mast cell responses has not yet been elucidated. In this study FcεRIγ-/- mast cells were reconstituted with either wild-type or mutant FcεRIγ in transgenic mice and transfected mast cells in vitro. We demonstrate that FcεRIγ-immunoreceptor tyrosine-based activation motif is essential for degranulation, cytokine production, and PG synthesis as well as for passive systemic anaphylaxis. Recent reports have suggested that cell surface FcεRI expression and mast cell survival are regulated by IgE in the absence of Ag, although the molecular mechanism is largely unknown. We also found that the promotion of mast cell survival by IgE without Ags is mediated by signals through the FcεRIγ-immunoreceptor tyrosine-based activation motif. In contrast, the IgE-mediated up-regulation of FcεRI is independent of FcεRIγ signaling. These results indicate that FcεRIγ-mediated signals differentially regulate the receptor expression, activation, and survival of mast cells and systemic anaphylaxis.

    本文言語英語
    ページ(範囲)2374-2381
    ページ数8
    ジャーナルJournal of Immunology
    172
    4
    DOI
    出版ステータス出版済み - 2月 15 2004

    !!!All Science Journal Classification (ASJC) codes

    • 免疫アレルギー学
    • 免疫学

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