Feedback regulation of β-thujaplicin production and formation of its methyl ether in a suspension culture of Cupressus lusitanica

Junko Yamada, Koki Fujita, Kokki Sakai

研究成果: ジャーナルへの寄稿学術誌査読

4 被引用数 (Scopus)

抄録

Suspension cell cultures of Cupressus lusitanica produce β-thujaplicin, a tropolone found mostly in Cupressaceae heartwood. The factors controlling β-thujaplicin accumulation in this cell culture system were investigated. Initial cell density of the cultures did not affect β-thujaplicin levels, though initial addition of β-thujaplicin suppressed its de novo production. When β-thujaplicin accumulation reached a certain level (ca. 40 mg/l) in the medium, the cultures seemed to cease β-thujaplicin production. However, β-thujaplicin productivity was restored when the β-thujaplicin-containing medium was exchanged for fresh medium; the formation of 2-methoxy-6-(methylethyl)cyclohepta-2,4,6-trien-1-one, an isomer of methylated β-thujaplicin, in medium was also observed. These results suggest that β-thujaplicin synthesis was regulated by product feedback mechanism in this cell line, and that excess accumulation of β-thujaplicin is relieved by conversion of β-thujaplicin to its methyl ether.

本文言語英語
ページ(範囲)447-450
ページ数4
ジャーナルPhytochemistry
60
5
DOI
出版ステータス出版済み - 7月 1 2002

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 植物科学
  • 園芸学

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