Feedback regulation of β-thujaplicin production and formation of its methyl ether in a suspension culture of Cupressus lusitanica

Junko Yamada, Koki Fujita, Kokki Sakai

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

Suspension cell cultures of Cupressus lusitanica produce β-thujaplicin, a tropolone found mostly in Cupressaceae heartwood. The factors controlling β-thujaplicin accumulation in this cell culture system were investigated. Initial cell density of the cultures did not affect β-thujaplicin levels, though initial addition of β-thujaplicin suppressed its de novo production. When β-thujaplicin accumulation reached a certain level (ca. 40 mg/l) in the medium, the cultures seemed to cease β-thujaplicin production. However, β-thujaplicin productivity was restored when the β-thujaplicin-containing medium was exchanged for fresh medium; the formation of 2-methoxy-6-(methylethyl)cyclohepta-2,4,6-trien-1-one, an isomer of methylated β-thujaplicin, in medium was also observed. These results suggest that β-thujaplicin synthesis was regulated by product feedback mechanism in this cell line, and that excess accumulation of β-thujaplicin is relieved by conversion of β-thujaplicin to its methyl ether.

元の言語英語
ページ(範囲)447-450
ページ数4
ジャーナルPhytochemistry
60
発行部数5
DOI
出版物ステータス出版済み - 7 1 2002

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tropolone
Cupressus lusitanica
Cupressus
Methyl Ethers
Cupressaceae
heartwood
Cell culture
cell suspension culture
isomers
ethers
Suspensions
cell culture
Cell Culture Techniques
Tropolone
culture media
cell lines
Trientine
Feedback
synthesis
Isomers

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

これを引用

Feedback regulation of β-thujaplicin production and formation of its methyl ether in a suspension culture of Cupressus lusitanica. / Yamada, Junko; Fujita, Koki; Sakai, Kokki.

:: Phytochemistry, 巻 60, 番号 5, 01.07.2002, p. 447-450.

研究成果: ジャーナルへの寄稿記事

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abstract = "Suspension cell cultures of Cupressus lusitanica produce β-thujaplicin, a tropolone found mostly in Cupressaceae heartwood. The factors controlling β-thujaplicin accumulation in this cell culture system were investigated. Initial cell density of the cultures did not affect β-thujaplicin levels, though initial addition of β-thujaplicin suppressed its de novo production. When β-thujaplicin accumulation reached a certain level (ca. 40 mg/l) in the medium, the cultures seemed to cease β-thujaplicin production. However, β-thujaplicin productivity was restored when the β-thujaplicin-containing medium was exchanged for fresh medium; the formation of 2-methoxy-6-(methylethyl)cyclohepta-2,4,6-trien-1-one, an isomer of methylated β-thujaplicin, in medium was also observed. These results suggest that β-thujaplicin synthesis was regulated by product feedback mechanism in this cell line, and that excess accumulation of β-thujaplicin is relieved by conversion of β-thujaplicin to its methyl ether.",
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N2 - Suspension cell cultures of Cupressus lusitanica produce β-thujaplicin, a tropolone found mostly in Cupressaceae heartwood. The factors controlling β-thujaplicin accumulation in this cell culture system were investigated. Initial cell density of the cultures did not affect β-thujaplicin levels, though initial addition of β-thujaplicin suppressed its de novo production. When β-thujaplicin accumulation reached a certain level (ca. 40 mg/l) in the medium, the cultures seemed to cease β-thujaplicin production. However, β-thujaplicin productivity was restored when the β-thujaplicin-containing medium was exchanged for fresh medium; the formation of 2-methoxy-6-(methylethyl)cyclohepta-2,4,6-trien-1-one, an isomer of methylated β-thujaplicin, in medium was also observed. These results suggest that β-thujaplicin synthesis was regulated by product feedback mechanism in this cell line, and that excess accumulation of β-thujaplicin is relieved by conversion of β-thujaplicin to its methyl ether.

AB - Suspension cell cultures of Cupressus lusitanica produce β-thujaplicin, a tropolone found mostly in Cupressaceae heartwood. The factors controlling β-thujaplicin accumulation in this cell culture system were investigated. Initial cell density of the cultures did not affect β-thujaplicin levels, though initial addition of β-thujaplicin suppressed its de novo production. When β-thujaplicin accumulation reached a certain level (ca. 40 mg/l) in the medium, the cultures seemed to cease β-thujaplicin production. However, β-thujaplicin productivity was restored when the β-thujaplicin-containing medium was exchanged for fresh medium; the formation of 2-methoxy-6-(methylethyl)cyclohepta-2,4,6-trien-1-one, an isomer of methylated β-thujaplicin, in medium was also observed. These results suggest that β-thujaplicin synthesis was regulated by product feedback mechanism in this cell line, and that excess accumulation of β-thujaplicin is relieved by conversion of β-thujaplicin to its methyl ether.

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