Feeder cells support the culture of induced pluripotent stem cells even after chemical fixation

Xiao Shan Yue, Masako Fujishiro, Chieko Nishioka, Takashi Arai, Eiki Takahashi, Jian Sheng Gong, Toshihiro Akaike, Yoshihiro Ito

研究成果: Contribution to journalArticle査読

29 被引用数 (Scopus)

抄録

Chemically fixed mouse embryonic fibroblasts (MEFs), instead of live feeder cells, were applied to the maintenance of mouse induced pluripotent stem (miPS) cells. Formaldehyde and glutaraldehyde were used for chemical fixation. The chemically fixed MEF feeders maintained the pluripotency of miPS cells, as well as their undifferentiated state. Furthermore, the chemically fixed MEF feeders were reused several times without affecting their functions. These results indicate that chemical fixation can be applied to modify biological feeders chemically, without losing their original functions. Chemically fixed MEF feeders will be applicable to other stem cell cultures as a reusable extracellular matrix candidate that can be preserved on a long-term basis.

本文言語英語
論文番号e32707
ジャーナルPloS one
7
3
DOI
出版ステータス出版済み - 3 2 2012
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)
  • 農業および生物科学(全般)
  • 一般

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