Fermentative production of P(3HB-co-3HV) from propionic acid by Alcaligenes eutrophus in fed-batch culture with pH-stat continuous substrate feeding method

Genta Kobayashi, Kuniaki Tanaka, Hirokazu Itoh, Takeharu Tsuge, Kenji Sonomoto, Ayaaki Ishizaki

研究成果: Contribution to journalArticle査読

23 被引用数 (Scopus)

抄録

The feeding of propionic acid for production of poly(3-hydroxybutyrate- co-3-hydroxyvalerate) [P(3HB-co-3HV)] by Alcaligenes eutrophus ATCC17697 was optimized using a fed-batch culture system. The concentration of propionic acid was maintained at 3 g l-1 as growth was inhibited by propionic acid in the broth. A pH-stat substrate feeding system was used in which propionic acid was fed automatically to maintain a pH of the culture broth at 7.0. By feeding a substrate solution containing 20% (w/v) propionic acid. 4.9% (w/v) ammonia water [at a molar ratio of carbon to nitrogen (C/N molar ratio) of 10] in cell growth phase, the concentration of propionic acid in the broth was maintained at 3 g l-1 giving a specific growth rate of 0.4 h-1. To promote P(3HB-co-3HV) production, two stage fed-batch culture which consisted of the stage for the cell growth and the stage for the P(3HB-co-3HV) accumulation was carried out. When the substrate solution whose C/N molar ratio was 50 was fed in P(3HB-co-3HV) accumulation phase, the cell concentration and the P(3HB-co-3HV) content in the cells reached 64 g l-1 and 58% (w/w) in 55.5 h, respectively.

本文言語英語
ページ(範囲)1067-1069
ページ数3
ジャーナルBiotechnology letters
22
13
DOI
出版ステータス出版済み - 2000

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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