Fibrosis in Preeclamptic Placentas Is Associated with Stromal Fibroblasts Activated by the Transforming Growth Factor-β1 Signaling Pathway

Takako Ohmaru-Nakanishi, Kazuo Asanoma, Mai Fujikawa, Yasuyuki Fujita, hiroshi yagi, Ichiro Onoyama, Nobuhiro Hidaka, Kenzo Sonoda, Kiyoko Kato

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

Although fibrosis is one of the most prominent pathologic features of preeclamptic (PE) placentas, its mechanism remains largely unknown. Consistent with previous reports, we observed overexpression of collagen; actin, α2, smooth muscle, aorta; connective tissue growth factor; and fibronectin in PE placentas compared with control ones. To investigate the mechanism of fibrosis in PE placentas, placental fibroblasts were isolated from PE placentas or normal pregnancies at delivery. The expression of fibrosis-related factors in fibroblasts was evaluated by real-time RT-PCR, Western blotting, enzyme-linked immunosorbent assay, and gene microarrays. An in vitro collagen gel contraction assay was also performed. Fibroblasts isolated from PE placentas showed higher expression levels of fibrosis-related factors compared with those from control ones. Global gene expression profiling of PE fibroblasts was contrasted with that of control ones and indicated an intimate association with transforming growth factor-β1 (TGFB1) signaling. Furthermore, the PE fibroblasts expressed abundant phosphorylated SMAD family member 2 and showed higher expression levels of target genes of TGFB1 signaling compared with the control ones. The PE fibroblasts also had a greater ability to contract compared with the control ones. Contractility also depended on TGFB1 signaling. Our results suggest that TGFB1 signaling is activated in the fibroblasts in PE placentas and that these active fibroblasts contribute to fibrosis.

元の言語英語
ページ(範囲)683-695
ページ数13
ジャーナルAmerican Journal of Pathology
188
発行部数3
DOI
出版物ステータス出版済み - 3 1 2018

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Transforming Growth Factors
Placenta
Fibrosis
Fibroblasts
Collagen
Connective Tissue Growth Factor
Aptitude
Gene Expression Profiling
Fibronectins
Genes
Smooth Muscle
Aorta
Actins
Real-Time Polymerase Chain Reaction
Western Blotting
Gels
Enzyme-Linked Immunosorbent Assay
Pregnancy

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

これを引用

Fibrosis in Preeclamptic Placentas Is Associated with Stromal Fibroblasts Activated by the Transforming Growth Factor-β1 Signaling Pathway. / Ohmaru-Nakanishi, Takako; Asanoma, Kazuo; Fujikawa, Mai; Fujita, Yasuyuki; yagi, hiroshi; Onoyama, Ichiro; Hidaka, Nobuhiro; Sonoda, Kenzo; Kato, Kiyoko.

:: American Journal of Pathology, 巻 188, 番号 3, 01.03.2018, p. 683-695.

研究成果: ジャーナルへの寄稿記事

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