Fidelity and processivity of DNA synthesis by DNA polymerase κ, the product of the human DINB1 gene

Eiji Ohashi, Katarzyna Bebenek, Toshiro Matsuda, William J. Feaver, Valerie L. Gerlach, Errol C. Friedberg, Haruo Ohmori, Thomas A. Kunkel

研究成果: Contribution to journalArticle査読

213 被引用数 (Scopus)

抄録

Mammalian DNA polymerase κ (pol κ), a member of the UmuC/DinB nucleotidyl transferase superfamily, has been implicated in spontaneous mutagenesis. Here we show that human pol κ copies undamaged DNA with average single-base substitution and deletion error rates of 7 × 10-3 and 2 × 10-3, respectively. These error rates are high when compared to those of most other DNA polymerases, pol κ also has unusual error specificity, producing a high proportion of T·CMP mispairs and deleting and adding non-reiterated nucleotides at extraordinary rates. Unlike other members of the UmuC/ DinB family, pol κ can processively synthesize chains of 25 or more nucleotides. This moderate processivity may reflect a contribution of C-terminal residues, which include two zinc clusters. The very low fidelity and moderate processivity of pol κ is novel in comparison to any previously studied DNA polymerase, and is consistent with a role in spontaneous mutagenesis.

本文言語英語
ページ(範囲)39678-39684
ページ数7
ジャーナルJournal of Biological Chemistry
275
50
DOI
出版ステータス出版済み - 12 15 2000
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

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