FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction

Hiroshi Nonaka, Sho hei Fujishima, Shohei Uchinomiya, Akio Ojida, Itaru Hamachi

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

抄録

A FLAG tag selective protein labeling method is newly developed in this study. Coupling of the selective binding between synthetic Ni-complex probe and FLAG tag with the acyl transfer reaction enables the site-selective covalent modification of FLAG peptide and FLAG-tag fused protein.

元の言語英語
ページ(範囲)6696-6699
ページ数4
ジャーナルBioorganic and Medicinal Chemistry Letters
19
発行部数23
DOI
出版物ステータス出版済み - 12 1 2009
外部発表Yes

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Labeling
Proteins
FLAG peptide

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmaceutical Science
  • Drug Discovery
  • Clinical Biochemistry
  • Organic Chemistry

これを引用

FLAG-tag selective covalent protein labeling via a binding-induced acyl-transfer reaction. / Nonaka, Hiroshi; Fujishima, Sho hei; Uchinomiya, Shohei; Ojida, Akio; Hamachi, Itaru.

:: Bioorganic and Medicinal Chemistry Letters, 巻 19, 番号 23, 01.12.2009, p. 6696-6699.

研究成果: ジャーナルへの寄稿記事

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AU - Hamachi, Itaru

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