Flavin-containing monooxygenases from Phanerochaete chrysosporium responsible for fungal metabolism of phenolic compounds

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

We investigated the cellular responses of the white-rot basidiomycete Phanerochaete chrysosporium against vanillin. Based upon a proteomic survey, it was demonstrated that two flavin-containing monooxygenases (PcFMO1 and PcFMO2) are translationally up-regulated in response to exogenous addition of vanillin. To elucidate their catalytic functions, we cloned cDNAs and heterologously expressed them in Escherichia coli. The recombinant PcFMO1 showed catalytic activities against monocyclic phenols such as phenol, hydroquinone, and 4-chlorophenol. In addition, the product from hydroquinone was identified as 1,2,4-trihydroxybenzene, an important intermediate in a metabolic pathway of aromatic compounds in which the aromatic ring of 1,2,4-trihydroxybenzene can be further cleaved by fungal dioxygenases for mineralization. Thus, the ortho-cleavage pathway of phenolic compounds would presumably be associated with PcFMO1.

元の言語英語
ページ(範囲)343-350
ページ数8
ジャーナルBiodegradation
23
発行部数3
DOI
出版物ステータス出版済み - 6 1 2012

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dimethylaniline monooxygenase (N-oxide forming)
Phanerochaete
phenolic compound
Metabolism
Phenols
phenol
metabolism
Dioxygenases
Basidiomycota
proteomics
Aromatic compounds
chlorophenol
Metabolic Networks and Pathways
Phenol
Proteomics
Escherichia coli
cleavage
Catalyst activity
Complementary DNA
mineralization

All Science Journal Classification (ASJC) codes

  • Environmental Engineering
  • Microbiology
  • Bioengineering
  • Environmental Chemistry
  • Pollution

これを引用

Flavin-containing monooxygenases from Phanerochaete chrysosporium responsible for fungal metabolism of phenolic compounds. / Nakamura, Tomofumi; Ichinose, Hirofumi; Wariishi, Hiroyuki.

:: Biodegradation, 巻 23, 番号 3, 01.06.2012, p. 343-350.

研究成果: ジャーナルへの寄稿記事

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