Flow-cytometric separation and enrichment of hepatic progenitor cells in the developing mouse liver

Atsushi Suzuki, Yun Wen Zheng, Reika Kondo, Moriaki Kusakabe, Yasutsugu Takada, Katashi Fukao, Hiromitsu Nakauchi, Hideki Taniguchi

研究成果: ジャーナルへの寄稿記事

266 引用 (Scopus)

抄録

Stem cells responsible for tissue maintenance and repair are found in a number of organs. However, hepatic stem cells assumed to play a key role in liver development and regeneration remain to be well characterized. To address this issue, we set up a culture system in which primitive hepatic progenitor cells formed colonies. By combining this culture system with fluorescence-activated cell sorting (FACS), cells forming colonies containing distinct hepatocytes and cholangiocytes were identified in the fetal mouse liver. These cells express both CD49f and CD29 (α6 and β1 integrin subunits), but do not mark for hematopoietic antigens such as CD45, TER11g, and c-Kit. When transplanted into the spleen, these cells migrated to the recipient liver and differentiated into liver parenchymal cells. Our data demonstrate that hepatic progenitor cells are enriched by FACS and suggest approaches to supplanting organ allografting and improving artificial-organ hepatic support.

元の言語英語
ページ(範囲)1230-1239
ページ数10
ジャーナルHepatology
32
発行部数6
DOI
出版物ステータス出版済み - 1 1 2000

Fingerprint

Hepatocytes
Stem Cells
Liver
Integrin alpha6beta1
Flow Cytometry
Artificial Organs
Liver Regeneration
Homologous Transplantation
Integrins
Spleen
Maintenance
Antigens

All Science Journal Classification (ASJC) codes

  • Hepatology

これを引用

Suzuki, A., Zheng, Y. W., Kondo, R., Kusakabe, M., Takada, Y., Fukao, K., ... Taniguchi, H. (2000). Flow-cytometric separation and enrichment of hepatic progenitor cells in the developing mouse liver. Hepatology, 32(6), 1230-1239. https://doi.org/10.1053/jhep.2000.20349

Flow-cytometric separation and enrichment of hepatic progenitor cells in the developing mouse liver. / Suzuki, Atsushi; Zheng, Yun Wen; Kondo, Reika; Kusakabe, Moriaki; Takada, Yasutsugu; Fukao, Katashi; Nakauchi, Hiromitsu; Taniguchi, Hideki.

:: Hepatology, 巻 32, 番号 6, 01.01.2000, p. 1230-1239.

研究成果: ジャーナルへの寄稿記事

Suzuki, A, Zheng, YW, Kondo, R, Kusakabe, M, Takada, Y, Fukao, K, Nakauchi, H & Taniguchi, H 2000, 'Flow-cytometric separation and enrichment of hepatic progenitor cells in the developing mouse liver', Hepatology, 巻. 32, 番号 6, pp. 1230-1239. https://doi.org/10.1053/jhep.2000.20349
Suzuki, Atsushi ; Zheng, Yun Wen ; Kondo, Reika ; Kusakabe, Moriaki ; Takada, Yasutsugu ; Fukao, Katashi ; Nakauchi, Hiromitsu ; Taniguchi, Hideki. / Flow-cytometric separation and enrichment of hepatic progenitor cells in the developing mouse liver. :: Hepatology. 2000 ; 巻 32, 番号 6. pp. 1230-1239.
@article{f964ca2189844070b4a7dcb8bb6f7324,
title = "Flow-cytometric separation and enrichment of hepatic progenitor cells in the developing mouse liver",
abstract = "Stem cells responsible for tissue maintenance and repair are found in a number of organs. However, hepatic stem cells assumed to play a key role in liver development and regeneration remain to be well characterized. To address this issue, we set up a culture system in which primitive hepatic progenitor cells formed colonies. By combining this culture system with fluorescence-activated cell sorting (FACS), cells forming colonies containing distinct hepatocytes and cholangiocytes were identified in the fetal mouse liver. These cells express both CD49f and CD29 (α6 and β1 integrin subunits), but do not mark for hematopoietic antigens such as CD45, TER11g, and c-Kit. When transplanted into the spleen, these cells migrated to the recipient liver and differentiated into liver parenchymal cells. Our data demonstrate that hepatic progenitor cells are enriched by FACS and suggest approaches to supplanting organ allografting and improving artificial-organ hepatic support.",
author = "Atsushi Suzuki and Zheng, {Yun Wen} and Reika Kondo and Moriaki Kusakabe and Yasutsugu Takada and Katashi Fukao and Hiromitsu Nakauchi and Hideki Taniguchi",
year = "2000",
month = "1",
day = "1",
doi = "10.1053/jhep.2000.20349",
language = "English",
volume = "32",
pages = "1230--1239",
journal = "Hepatology",
issn = "0270-9139",
publisher = "John Wiley and Sons Ltd",
number = "6",

}

TY - JOUR

T1 - Flow-cytometric separation and enrichment of hepatic progenitor cells in the developing mouse liver

AU - Suzuki, Atsushi

AU - Zheng, Yun Wen

AU - Kondo, Reika

AU - Kusakabe, Moriaki

AU - Takada, Yasutsugu

AU - Fukao, Katashi

AU - Nakauchi, Hiromitsu

AU - Taniguchi, Hideki

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Stem cells responsible for tissue maintenance and repair are found in a number of organs. However, hepatic stem cells assumed to play a key role in liver development and regeneration remain to be well characterized. To address this issue, we set up a culture system in which primitive hepatic progenitor cells formed colonies. By combining this culture system with fluorescence-activated cell sorting (FACS), cells forming colonies containing distinct hepatocytes and cholangiocytes were identified in the fetal mouse liver. These cells express both CD49f and CD29 (α6 and β1 integrin subunits), but do not mark for hematopoietic antigens such as CD45, TER11g, and c-Kit. When transplanted into the spleen, these cells migrated to the recipient liver and differentiated into liver parenchymal cells. Our data demonstrate that hepatic progenitor cells are enriched by FACS and suggest approaches to supplanting organ allografting and improving artificial-organ hepatic support.

AB - Stem cells responsible for tissue maintenance and repair are found in a number of organs. However, hepatic stem cells assumed to play a key role in liver development and regeneration remain to be well characterized. To address this issue, we set up a culture system in which primitive hepatic progenitor cells formed colonies. By combining this culture system with fluorescence-activated cell sorting (FACS), cells forming colonies containing distinct hepatocytes and cholangiocytes were identified in the fetal mouse liver. These cells express both CD49f and CD29 (α6 and β1 integrin subunits), but do not mark for hematopoietic antigens such as CD45, TER11g, and c-Kit. When transplanted into the spleen, these cells migrated to the recipient liver and differentiated into liver parenchymal cells. Our data demonstrate that hepatic progenitor cells are enriched by FACS and suggest approaches to supplanting organ allografting and improving artificial-organ hepatic support.

UR - http://www.scopus.com/inward/record.url?scp=0033659178&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033659178&partnerID=8YFLogxK

U2 - 10.1053/jhep.2000.20349

DO - 10.1053/jhep.2000.20349

M3 - Article

C2 - 11093729

AN - SCOPUS:0033659178

VL - 32

SP - 1230

EP - 1239

JO - Hepatology

JF - Hepatology

SN - 0270-9139

IS - 6

ER -