Fluctuation in the microtubule sliding movement driven by kinesin in vitro

Yasuhiro Imafuku, Yoko Y. Toyoshima, Katsuhisa Tawada

研究成果: ジャーナルへの寄稿記事

26 引用 (Scopus)

抄録

We studied the fluctuation in the translational sliding movement of microtubules driven by kinesin in a motility assay in vitro. By calculating the mean-square displacement deviation from the average as a function of time, we obtained motional diffusion coefficients for microtubules and analyzed the dependence of the coefficients on microtubule length. Our analyses suggest that 1) the motional diffusion coefficient consists of the sum of two terms, one that is proportional to the inverse of the microtubule length (as the longitudinal diffusion coefficient of a filament in Brownian movement is) and another that is independent of the length, and 2) the length-dependent term decreases with increasing kinesin concentration. This latter term almost vanishes within the length range we studied at high kinesin concentrations. From the length-dependence relationship, we evaluated the friction coefficient for sliding microtubules. This value is much larger than the solvent friction and thus consistent with protein friction. The length independence of the motional diffusion coefficient observed at sufficiently high kinesin concentrations indicates the presence of correlation in the sliding movement fluctuation. This places significant constraint on the possible mechanisms of the sliding movement generation by kinesin motors in vitro.

元の言語英語
ページ(範囲)878-886
ページ数9
ジャーナルBiophysical Journal
70
発行部数2 I
DOI
出版物ステータス出版済み - 1 1 1996

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Kinesin
Microtubules
Friction
In Vitro Techniques
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics

これを引用

Fluctuation in the microtubule sliding movement driven by kinesin in vitro. / Imafuku, Yasuhiro; Toyoshima, Yoko Y.; Tawada, Katsuhisa.

:: Biophysical Journal, 巻 70, 番号 2 I, 01.01.1996, p. 878-886.

研究成果: ジャーナルへの寄稿記事

Imafuku, Yasuhiro ; Toyoshima, Yoko Y. ; Tawada, Katsuhisa. / Fluctuation in the microtubule sliding movement driven by kinesin in vitro. :: Biophysical Journal. 1996 ; 巻 70, 番号 2 I. pp. 878-886.
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