Fluorescence imaging of intracellular cadmium using a dual-excitation ratiometric chemosensor

Masayasu Taki, Mika Desaki, Akio Ojida, Shohei Iyoshi, Tasuku Hirayama, Itaru Hamachi, Yukio Yamamoto

研究成果: ジャーナルへの寄稿記事

175 引用 (Scopus)

抄録

We described here a coumarin-based dual-excitation ratiometric probe for cadmium, CadMQ. This fluorescence sensor has high quantum yields of 0.59 and 0.70 in the metal-free and Cd2+-bound forms, respectively, and has a dissociation constant of 0.16 nM for Cd2+. CadMQ is cell permeable and locates within the acidic compartments of the cells. We further show that CadMQ is a useful tool to ratiometrically probe the change in the intracellular Cd2+ levels with the use of two excited wavelengths.

元の言語英語
ページ(範囲)12564-12565
ページ数2
ジャーナルJournal of the American Chemical Society
130
発行部数38
DOI
出版物ステータス出版済み - 9 24 2008
外部発表Yes

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Optical Imaging
Cadmium
Fluorescence
Imaging techniques
Quantum yield
Metals
Wavelength
Sensors
coumarin

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

これを引用

Fluorescence imaging of intracellular cadmium using a dual-excitation ratiometric chemosensor. / Taki, Masayasu; Desaki, Mika; Ojida, Akio; Iyoshi, Shohei; Hirayama, Tasuku; Hamachi, Itaru; Yamamoto, Yukio.

:: Journal of the American Chemical Society, 巻 130, 番号 38, 24.09.2008, p. 12564-12565.

研究成果: ジャーナルへの寄稿記事

Taki, M, Desaki, M, Ojida, A, Iyoshi, S, Hirayama, T, Hamachi, I & Yamamoto, Y 2008, 'Fluorescence imaging of intracellular cadmium using a dual-excitation ratiometric chemosensor', Journal of the American Chemical Society, 巻. 130, 番号 38, pp. 12564-12565. https://doi.org/10.1021/ja803429z
Taki, Masayasu ; Desaki, Mika ; Ojida, Akio ; Iyoshi, Shohei ; Hirayama, Tasuku ; Hamachi, Itaru ; Yamamoto, Yukio. / Fluorescence imaging of intracellular cadmium using a dual-excitation ratiometric chemosensor. :: Journal of the American Chemical Society. 2008 ; 巻 130, 番号 38. pp. 12564-12565.
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AU - Hirayama, Tasuku

AU - Hamachi, Itaru

AU - Yamamoto, Yukio

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