Fluorescence microscopic imaging using a substrate with a sub-wavelength grating

Keiko Tawa, Hironobu Hori, Yoshiro Tatsu, Junji Nishii, Kenji Kintaka, Kazuyuki Kiyosue

研究成果: Contribution to journalShort survey査読

抄録

A study was conducted to demonstrate fluorescent microscopic imaging using a substrate with a sub-wavelength grating. Enhanced fluorescence detection was carried out using grating-coupled (GC)-surface plasmon resonance (SPR) to overcome the challenges associated with conventional prism coupled SPR-design. A thin metal coated grating substrate with a rectangular or trapezoidal surface profile in GC-SPR allowed the resonance angle to be reduced. The periodic structure was fabricated using two-beam interference and dry etching or UV-nanoprint lithography. It was also demonstrated that a periodical structure of 400 nm created on the substrate surface was covered with Ag and SiO 2 layers to be used as a chip.

本文言語英語
ページ(範囲)9-10
ページ数2
ジャーナルAIST Today (International Edition)
34
出版ステータス出版済み - 2009
外部発表はい

All Science Journal Classification (ASJC) codes

  • 工学(全般)

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