Fluorescence study of the high pressure-induced denaturation of skeletal muscle actin

Yoshihide Ikeuchi, Atsusi Suzuki, Takayoshi Oota, Kazuaki Hagiwara, Ryuichi Tatsumi, Tatsumi Ito, Claude Balny

研究成果: Contribution to journalArticle査読

22 被引用数 (Scopus)


Ikkai & Ooi [Ikkai, T. & Ooi, T. (1966) Biochemistry 5, 1551-1560] made a thorough study of the effect of pressure on G- and F-actins. However, all of the measurements in their study were made after the release of pressure. In the present experiment in situ observations were attempted by using εATP to obtain further detailed kinetic and thermodynamic information about the behaviour of actin under pressure. The dissociation rate constants of nucleotides from actin molecules (the decay curve of the intensity of fluorescence of εATP-G-actin or εADP-F-actin) followed first-order kinetics. The volume changes forthe denaturation of G-actin and F-actin were estimated to be -72 mL·mol-1 and -67 mL·mol-1 in the presence of ATP, respectively. Changes in the intensity of fluorescence of F-actin whilst under pressure suggested that εADP-F-actin was initially depolymerized to εADP-G-actin; subsequently there was quick exchange of the εADP for free εATP, and then polymerization occurred again with the liberation of phosphate from εATP bound to G-actin in the presence of excess ATP. In the higher pressure range (> 250 MPa), the partial collapse of the three-dimensional structure of actin, which had been depolymerized under pressure, proceeded immediately after release of the nucleotide, so that it lost the ability to exchange bound ADP with external free ATP and so was denatured irreversibly. An experiment monitoring εATP fluorescence also demonstrated that, in the absence of Mg2+-ATP, the dissociation of actin-heavy meromyosin (HMM) complex into actin and HMM did not occur under high pressure.

ジャーナルEuropean Journal of Biochemistry
出版ステータス出版済み - 2002

All Science Journal Classification (ASJC) codes

  • Biochemistry

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