Fluorescent detection of target proteins: Via a molecularly imprinted hydrogel

Takuya Kubo, Naoki Watanabe, Seiji Ikari, Chenchen Liu, Eisuke Kanao, Toyohiro Naito, Tomoharu Sano, Koji Otsuka

研究成果: Contribution to journalArticle査読

抄録

Proteins are typically separated by an immune reaction, such as an enzyme-linked immunosorbent assay, and are detected by selective fluorescent labeling. This has potential for complicated procedures and the denaturation of proteins by labeling, and is cost consuming. In this study, we propose a technique for the selective separation and detection of a target protein using a molecularly imprinted hydrogel (PI gel) with fluorescent monomers. We focused on 8-anilino-1-naphthalenesulfonic acid (ANS), where the fluorescence intensity is easily changed by the interaction with proteins, and successfully synthesized the ANS monomer and a poly(ethylene glycol) (PEG) conjugated ANS monomer. The PI gel with the ANS monomers using bovine serum albumin (BSA) as a template showed the selective adsorption of BSA and the fluorescence intensity increased due to the adsorption of BSA.

本文言語英語
ページ(範囲)3086-3091
ページ数6
ジャーナルAnalytical Methods
13
27
DOI
出版ステータス出版済み - 7 21 2021

All Science Journal Classification (ASJC) codes

  • 分析化学
  • 化学工学(全般)
  • 工学(全般)

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