Fluorescent differential display analysis of gene expression in apoptotic neuroblastoma cells

Dong Kug Choi, Takashi Ito, Yuko Mitsui, Yoshiyuki Sakaki

研究成果: ジャーナルへの寄稿記事

39 引用 (Scopus)

抄録

Identification of differentially expressed genes will provide leads in the elucidation of the molecular mechanisms underlying neuronal cell death associated with neurodegenerative disorders. Using a high-throughput fluorescent differential display (FDD) system based on an automated DNA sequencer, we analyzed global patterns of gene expression during the apoptosis of neuroblastoma SH-SY5Y cells induced by a neurotoxin, colchicine. Initial screening of ~24 000 cDNA bands displayed with 320 primer combinations has revealed 263 fragments showing differential expression patterns, suggesting that ~1% of transcripts are modulated in their expression level. Of these differentially displayed bands, we cloned 18 fragments composed of 17 distinct species and confirmed differential expression of each species by reverse transcription-PCR or Northern blot hybridization, thereby proving the reliability of the approach. These include eight derived from seven known genes, five homologous to expressed sequence tags (ESTs), and five totally lacking any homology to those deposited in the database. Among these, a novel transcript SAIl induced prominently was characterized further and revealed to encode a putative RNA-binding protein NAPOR (neuroblastoma apoptosis-related RNA-binding protein), containing three copies of evolutionarily conserved RNA recognition motif. Since several RNA- binding proteins have been known to play crucial roles in other apoptosis systems, it is conceivable that NAPOR is also involved in the process of neuronal cell death.

元の言語英語
ページ(範囲)21-31
ページ数11
ジャーナルGene
223
発行部数1-2
DOI
出版物ステータス出版済み - 11 26 1998
外部発表Yes

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RNA-Binding Proteins
Neuroblastoma
Apoptosis
Gene Expression
Cell Death
Expressed Sequence Tags
Neurotoxins
Colchicine
Neurodegenerative Diseases
Northern Blotting
Genes
Reverse Transcription
Complementary DNA
Databases
Polymerase Chain Reaction
DNA

All Science Journal Classification (ASJC) codes

  • Genetics

これを引用

Fluorescent differential display analysis of gene expression in apoptotic neuroblastoma cells. / Choi, Dong Kug; Ito, Takashi; Mitsui, Yuko; Sakaki, Yoshiyuki.

:: Gene, 巻 223, 番号 1-2, 26.11.1998, p. 21-31.

研究成果: ジャーナルへの寄稿記事

Choi, Dong Kug ; Ito, Takashi ; Mitsui, Yuko ; Sakaki, Yoshiyuki. / Fluorescent differential display analysis of gene expression in apoptotic neuroblastoma cells. :: Gene. 1998 ; 巻 223, 番号 1-2. pp. 21-31.
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