Fluorescent labeling of a DNA sequencing primer

Junichi Mineno, Yoshizumi Ishino, Tadashi Ohminami, Ikunoshin Kato

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

Several oligonucleotides containing one to four fluorescein labels in various positions were synthesized and the fluorescence intensity and thermal stability of the duplex forms with their complementary sequences were measured. Oligomers that contain two fluorescein molecules, one attached at the 5' terminus and the other at an internal phosphate, were hybridized with less stability than that containing fluorescein at only the 5' terminus, but it formed more efficient primers for dideoxy sequencing with an automated sequencer.

本文言語英語
ページ(範囲)135-141
ページ数7
ジャーナルMitochondrial DNA
4
3
DOI
出版ステータス出版済み - 1 1 1993
外部発表はい

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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