Fluorescent polyion complex nanoparticle that incorporates an internal standard for quantitative analysis of protein kinase activity

Takanobu Nobori, Shujiro Shiosaki, Takeshi Mori, Riki Toita, Chan Woo Kim, Yuta Nakamura, Akihiro Kishimura, Takuro Niidome, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

We demonstrate a polyion complex (PIC) nanoparticle that contains both a responsive fluorophore and an "internal standard" fluorophore for quantitative measurement of protein kinase (PK) activity. The PK-responsive fluorophore becomes more fluorescent with PK-catalyzed phosphorylation of substrate peptides incorporated in the PIC, while fluorescence from the internal standard remains unchanged during phosphorylation. This new concept will be useful for quantitative PK assays and the discovery of PK inhibitors.

元の言語英語
ページ(範囲)869-872
ページ数4
ジャーナルBioconjugate Chemistry
25
発行部数5
DOI
出版物ステータス出版済み - 5 21 2014

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Nanoparticles
Protein Kinases
Fluorophores
Proteins
Phosphorylation
Chemical analysis
Protein Kinase Inhibitors
Assays
Fluorescence
Peptides
Substrates

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

これを引用

Fluorescent polyion complex nanoparticle that incorporates an internal standard for quantitative analysis of protein kinase activity. / Nobori, Takanobu; Shiosaki, Shujiro; Mori, Takeshi; Toita, Riki; Kim, Chan Woo; Nakamura, Yuta; Kishimura, Akihiro; Niidome, Takuro; Katayama, Yoshiki.

:: Bioconjugate Chemistry, 巻 25, 番号 5, 21.05.2014, p. 869-872.

研究成果: ジャーナルへの寄稿記事

Nobori, Takanobu ; Shiosaki, Shujiro ; Mori, Takeshi ; Toita, Riki ; Kim, Chan Woo ; Nakamura, Yuta ; Kishimura, Akihiro ; Niidome, Takuro ; Katayama, Yoshiki. / Fluorescent polyion complex nanoparticle that incorporates an internal standard for quantitative analysis of protein kinase activity. :: Bioconjugate Chemistry. 2014 ; 巻 25, 番号 5. pp. 869-872.
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AU - Kim, Chan Woo

AU - Nakamura, Yuta

AU - Kishimura, Akihiro

AU - Niidome, Takuro

AU - Katayama, Yoshiki

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