Folate receptor-specific cell-cell adhesion by using a folate-modified peptide-based anchor

Hiroko Nagai, Wataru Hatanaka, Masayoshi Matsuda, Akihiro Kishimura, Yoshiki Katayama, Takeshi Mori

研究成果: ジャーナルへの寄稿記事

抄録

We report here a folate-modified membrane anchor for cell surface modification to induce cell adhesion to target cells. The membrane anchor region, which was consisted of cationic lysine residues and palmitoyl group-modified residues, was modified with folate through an oligoethlene glycol linker. The peptide anchor was modified on to the cell membrane by using β-cyclodextrin as a solubilizer of the peptide anchor. After modification, the peptide anchor disappeared from the cell membrane via endocytotic uptake or dissociation from the cell membrane. However, the endocytosed peptide was represented on the cell surface via recycling endosome pathway. The obtained folate-modified cells successfully adhered on to target cells which expressed folate receptor α via ligand-receptor specific interaction and adhesion continued at least 4 hours.

元の言語英語
ページ(範囲)983-993
ページ数11
ジャーナルJournal of Biomaterials Science, Polymer Edition
30
発行部数11
DOI
出版物ステータス出版済み - 7 24 2019

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Cell adhesion
Anchors
Folic Acid
Cell Adhesion
Peptides
Cell Membrane
Cell membranes
Glycols
Endosomes
Cyclodextrins
Membranes
Endocytosis
Lysine
Surface treatment
Recycling
Ligands
Adhesion

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Biomedical Engineering

これを引用

Folate receptor-specific cell-cell adhesion by using a folate-modified peptide-based anchor. / Nagai, Hiroko; Hatanaka, Wataru; Matsuda, Masayoshi; Kishimura, Akihiro; Katayama, Yoshiki; Mori, Takeshi.

:: Journal of Biomaterials Science, Polymer Edition, 巻 30, 番号 11, 24.07.2019, p. 983-993.

研究成果: ジャーナルへの寄稿記事

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AU - Katayama, Yoshiki

AU - Mori, Takeshi

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