Formation of 20-oxoleukotriene B4 by an alcohol dehydrogenase isolated from human neutrophils

Yoichi Gotoh, Hideki Sumimoto, Shigeki Minakami

研究成果: Contribution to journalArticle査読

13 被引用数 (Scopus)

抄録

When 20-hydroxyleukotriene B4 (20-OH-LTB4) is incubated at pH 10.5 in the presence of NAD+ with an alcohol dehydrogenase isolated from human neutrophils, a polar product is formed as detected on reverse-phase high-performance liquid chromatography (RP-HPLC). The product is identified as 20-oxo-LTB4 (20-CHO-LTB4) on the basis of its co-elution with the authentic compound on HPLC, ultraviolet spectrometry and gas chromatography-mass spectrometry. The 20-CHO-LTB4-forming activity requires NAD+, but NADP+ scarcely replaces NAD+. The apparent Km for 20-OH-LTB4 is 83 μM and the Vmax is 2.04 μmol/min per mg of protein. The activity is inhibited by ω-hydroxy fatty acids such as 12-hydroxylauric acid, 16-hydroxypalmitic acid and 12(S), 20-dihydroxyeicosatetraenoic acid, but not by 4-methylpyrazole. At pH 7.0 with NADH, the purified dehydrogenase catalyzes the reverse reaction, the reduction of 20-CHO-LTB4 to 20-OH-LTB4.

本文言語英語
ページ(範囲)52-56
ページ数5
ジャーナルBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
1043
1
DOI
出版ステータス出版済み - 3 12 1990
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 内分泌学

フィンガープリント

「Formation of 20-oxoleukotriene B<sub>4</sub> by an alcohol dehydrogenase isolated from human neutrophils」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル