Formation of a spherical multicellular aggregate (spheroid) of animal cells in the pores of polyurethane foam as a cell culture substratum and its application to a hybrid artificial liver

Hiroyuki Ijima, Kohji Nakazawa, Hiroshi Mizumoto, Taku Matsushita, Kazumori Funatsu

研究成果: ジャーナルへの寄稿学術誌査読

82 被引用数 (Scopus)

抄録

Monkey kidney cells (Vero), human embryonic kidney cells (293), human liver cells (PLC/PRF/5), and primary rat, dog, and porcine hepatocytes formed spherical multicellular aggregates (spheroids) in the pores of polyurethane foam which was used as a cell culture substratum. These spheroids of various cell types express high cell activity for a long period. A practical hybrid artificial liver support system composed of a multi-capillary polyurethane foam packed-bed type cell culture module including primary hepatocyte spheroids was developed. The success of the system is indicated by an 80% recovery rate in hepatic failure rats which died in control experiments.

本文言語英語
ページ(範囲)765-778
ページ数14
ジャーナルJournal of Biomaterials Science, Polymer Edition
9
7
DOI
出版ステータス出版済み - 1月 1 1998

!!!All Science Journal Classification (ASJC) codes

  • 生物理学
  • バイオエンジニアリング
  • 生体材料
  • 生体医工学

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