Formation of covellite (CuS) under biological sulfate-reducing conditions

Jonathan P. Gramp, Keiko Sasaki, Jerry M. Bigham, Olia V. Karnachuk, Olli H. Tuovinen

研究成果: ジャーナルへの寄稿記事

27 引用 (Scopus)

抄録

Sulfate-reducing bacteria (SRB) play a major role in the precipitation of metal sulfides in the environment. In this work, biogenic copper sulfide formation was examined in cultures of SRB and compared to chemically initiated Cu sulfide precipitation as a reference system. Mixed cultures of SRB were incubated at 22, 45, and 60°C in nutrient solutions that contained copper sulfate. Abiotic reference samples were produced by reacting uninoculated liquid media with Na2S solutions under otherwise identical conditions. Precipitates were collected anaerobically by centrifugation, frozen in liquid N2, and freeze-dried, followed by analysis using X-ray diffraction (XRD), X-ray fluorescence, and scanning electron microscopy. Covellite (CuS) was the only mineral found in the precipitates. Covellite was less crystalline in the biogenic precipitates than in the abiotic samples based on XRD peak widths and peak to background ratios. Poor crystallinity may be the result of slower precipitation rates in bacterial cultures as compared to the abiotic reference systems. Furthermore, bacterial cells may inhibit the nucleation steps that lead to crystal formation. Incubation at elevated temperatures improved the crystallinity of the biotic specimens.

元の言語英語
ページ(範囲)613-619
ページ数7
ジャーナルGeomicrobiology Journal
23
発行部数8
DOI
出版物ステータス出版済み - 12 1 2006

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sulfate-reducing bacterium
Sulfides
Sulfates
Precipitates
Bacteria
sulfide
crystallinity
sulfate
X-Ray Diffraction
X-ray diffraction
copper
Copper Sulfate
reference sample
liquid
Centrifugation
Liquids
X-ray fluorescence
Electron Scanning Microscopy
X ray diffraction analysis
nucleation

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Environmental Chemistry
  • Environmental Science(all)
  • Earth and Planetary Sciences (miscellaneous)

これを引用

Formation of covellite (CuS) under biological sulfate-reducing conditions. / Gramp, Jonathan P.; Sasaki, Keiko; Bigham, Jerry M.; Karnachuk, Olia V.; Tuovinen, Olli H.

:: Geomicrobiology Journal, 巻 23, 番号 8, 01.12.2006, p. 613-619.

研究成果: ジャーナルへの寄稿記事

Gramp, Jonathan P. ; Sasaki, Keiko ; Bigham, Jerry M. ; Karnachuk, Olia V. ; Tuovinen, Olli H. / Formation of covellite (CuS) under biological sulfate-reducing conditions. :: Geomicrobiology Journal. 2006 ; 巻 23, 番号 8. pp. 613-619.
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abstract = "Sulfate-reducing bacteria (SRB) play a major role in the precipitation of metal sulfides in the environment. In this work, biogenic copper sulfide formation was examined in cultures of SRB and compared to chemically initiated Cu sulfide precipitation as a reference system. Mixed cultures of SRB were incubated at 22, 45, and 60°C in nutrient solutions that contained copper sulfate. Abiotic reference samples were produced by reacting uninoculated liquid media with Na2S solutions under otherwise identical conditions. Precipitates were collected anaerobically by centrifugation, frozen in liquid N2, and freeze-dried, followed by analysis using X-ray diffraction (XRD), X-ray fluorescence, and scanning electron microscopy. Covellite (CuS) was the only mineral found in the precipitates. Covellite was less crystalline in the biogenic precipitates than in the abiotic samples based on XRD peak widths and peak to background ratios. Poor crystallinity may be the result of slower precipitation rates in bacterial cultures as compared to the abiotic reference systems. Furthermore, bacterial cells may inhibit the nucleation steps that lead to crystal formation. Incubation at elevated temperatures improved the crystallinity of the biotic specimens.",
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N2 - Sulfate-reducing bacteria (SRB) play a major role in the precipitation of metal sulfides in the environment. In this work, biogenic copper sulfide formation was examined in cultures of SRB and compared to chemically initiated Cu sulfide precipitation as a reference system. Mixed cultures of SRB were incubated at 22, 45, and 60°C in nutrient solutions that contained copper sulfate. Abiotic reference samples were produced by reacting uninoculated liquid media with Na2S solutions under otherwise identical conditions. Precipitates were collected anaerobically by centrifugation, frozen in liquid N2, and freeze-dried, followed by analysis using X-ray diffraction (XRD), X-ray fluorescence, and scanning electron microscopy. Covellite (CuS) was the only mineral found in the precipitates. Covellite was less crystalline in the biogenic precipitates than in the abiotic samples based on XRD peak widths and peak to background ratios. Poor crystallinity may be the result of slower precipitation rates in bacterial cultures as compared to the abiotic reference systems. Furthermore, bacterial cells may inhibit the nucleation steps that lead to crystal formation. Incubation at elevated temperatures improved the crystallinity of the biotic specimens.

AB - Sulfate-reducing bacteria (SRB) play a major role in the precipitation of metal sulfides in the environment. In this work, biogenic copper sulfide formation was examined in cultures of SRB and compared to chemically initiated Cu sulfide precipitation as a reference system. Mixed cultures of SRB were incubated at 22, 45, and 60°C in nutrient solutions that contained copper sulfate. Abiotic reference samples were produced by reacting uninoculated liquid media with Na2S solutions under otherwise identical conditions. Precipitates were collected anaerobically by centrifugation, frozen in liquid N2, and freeze-dried, followed by analysis using X-ray diffraction (XRD), X-ray fluorescence, and scanning electron microscopy. Covellite (CuS) was the only mineral found in the precipitates. Covellite was less crystalline in the biogenic precipitates than in the abiotic samples based on XRD peak widths and peak to background ratios. Poor crystallinity may be the result of slower precipitation rates in bacterial cultures as compared to the abiotic reference systems. Furthermore, bacterial cells may inhibit the nucleation steps that lead to crystal formation. Incubation at elevated temperatures improved the crystallinity of the biotic specimens.

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