Formation of cylindrical multicellular aggregate (cylindroid) and expression of liver specific functions of primary rat hepatocytes

Hiroshi Mizumoto, Masashi Hayakami, Kohji Nakazawa, Hiroyuki Ijima, Kazumori Funatsu

研究成果: Contribution to journalArticle査読

11 被引用数 (Scopus)

抄録

In our studies of the development of a hybrid artificial liver, we investigated the formation of cylindrical multicellular aggregate (cylindroid) of primary rat hepatocytes on a pressed sheet of polyurethane foam (pressed-PUF) as a culture substratum. Hepatocytes formed cylindroids by attaching to a pressed-PUF surface, peeling off from the surface and aggregating. The diameter and length of most cylindroids were approximately 200-500 μm and 500 μm-2 mm, respectively. The activities of liver specific functions (albumin secretion and ammonia metabolism) of hepatocyte cylindroids were equivalent to or higher than those of hepatocyte spheroids. These results suggest that hepatocyte cylindroids can maintain highly differentiated functions longer than hepatocyte spheroids, and that a PUF/cylindroid culture may be effective to develop of a hybrid artificial liver.

本文言語英語
ページ(範囲)69-75
ページ数7
ジャーナルCytotechnology
31
1-2
DOI
出版ステータス出版済み - 1999

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 生体医工学
  • 臨床生化学
  • 細胞生物学

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