Formation of leukotriene B4-coenzyme a ester by rat liver microsomes

Atsushi Yamaoka, Hideki Sumimoto, Ryuichi Isobe, Shigeki Minakami

研究成果: Contribution to journalArticle査読

6 被引用数 (Scopus)

抄録

When leukotriene B4 (LTB4) was incubated with rat liver microsomal fraction in the presence of coenzyme A (CoA) and ATP, a more polar product (compound I) was detected on reverse-phase high-performance liquid chromatography (RP-HPLC). The product was identified as LTB4-CoA ester on the basis of ultraviolet spectrometry, alkaline hydrolysis followed by RP-HPLC, and fast atom bombardment mass spectrometry (FAB-MS). The activity forming LTB4-CoA ester was localized in the microsomal fraction. The reaction was proportional to the concentration of the microsomal protein with an optimal pH of 7.5-8.0 and completely dependent on CoA and ATP. Palmitic acid and myristic acid significantly inhibited the formation.

本文言語英語
ページ(範囲)1248-1252
ページ数5
ジャーナルBiochemical and Biophysical Research Communications
154
3
DOI
出版ステータス出版済み - 8 15 1988
外部発表はい

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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