Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization

Hiroyuki Tanaka; Noriko Fukuda; Yukihiro Shoyama

doi:10.1023/A:1008068718392

Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization

Hiroyuki Tanaka, Noriko Fukuda, Yukihiro Shoyama

生命薬学

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

63 被引用数 (Scopus)

抄録

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb₁ was produced by fusing splenocytes immunized with a ginsenoside Rb₁-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Rc and ginsenoside Rd. The full measuring range of the assay extends from 20 ngml^-1 to 400 ngml^-1 of ginsenoside Rb₁.

本文言語	英語
ページ（範囲）	115-120
ページ数	6
ジャーナル	Cytotechnology
巻	29
号	2
DOI	https://doi.org/10.1023/A:1008068718392
出版ステータス	出版済み - 1999

!!!All Science Journal Classification (ASJC) codes

バイオテクノロジー
バイオエンジニアリング
生体医工学
臨床生化学
細胞生物学

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10.1023/A:1008068718392

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title = "Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization",

abstract = "The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rb1 was produced by fusing splenocytes immunized with a ginsenoside Rb1-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Rc and ginsenoside Rd. The full measuring range of the assay extends from 20 ngml-1 to 400 ngml-1 of ginsenoside Rb1.",

author = "Hiroyuki Tanaka and Noriko Fukuda and Yukihiro Shoyama",

note = "Funding Information: The research in this paper was supported in part by a Grant-in-Aid from the Ministry of Education, Science and Culture of Japan.",

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doi = "10.1023/A:1008068718392",

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AU - Shoyama, Yukihiro

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Formation of monoclonal antibody against a major ginseng component, ginsenoside Rb1 and its characterization

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!!!All Science Journal Classification (ASJC) codes

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