Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization: Monoclonal antibody for a ginseng saponin

Noriko Fukuda, Hiroyuki Tanaka, Yukihiro Shoyama

研究成果: ジャーナルへの寄稿記事

48 引用 (Scopus)

抄録

The ratio of hapten and bovine serum albumin in an antigen conjugate was determined by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. A hybridoma secreting monoclonal antibody against ginsenoside Rg1 was produced by fusing sprenocytes immunized with a ginsenoside Rg1-bovine serum albumin conjugate with HAT-sensitive mouse myeloma cell line, P3-X63-Ag8-653. A very small cross-reaction appeared with ginsenoside Re. The full measuring range of the assay extends from 0.3 μg ml-1 to 10 μg ml-1 of ginsenoside Rg1.

元の言語英語
ページ(範囲)197-204
ページ数8
ジャーナルCytotechnology
34
発行部数3
DOI
出版物ステータス出版済み - 1 1 2000

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Panax
Monoclonal antibodies
Saponins
Antigens
Ionization
Mass spectrometry
Assays
Desorption
Monoclonal Antibodies
Cells
Bovine Serum Albumin
Lasers
Ginsenosides
Haptens
Cross Reactions
Hybridomas
Mass Spectrometry
Cell Line
ginsenoside Rg1

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

これを引用

Formation of monoclonal antibody against a major ginseng component, ginsenoside Rg1 and its characterization : Monoclonal antibody for a ginseng saponin. / Fukuda, Noriko; Tanaka, Hiroyuki; Shoyama, Yukihiro.

:: Cytotechnology, 巻 34, 番号 3, 01.01.2000, p. 197-204.

研究成果: ジャーナルへの寄稿記事

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