Formation of TiO2 nanostructures by enzyme-mediated self-assembly for the destruction of macrophages

Koichiro Hayashi, Michihiro Nakamura, Wataru Sakamoto, Toshinobu Yogo, Toshinari Kori, Kazunori Ishimura

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

Conifer foliage-like rutile TiO2 nanoparticles (CFR NPs), spherical anatase TiO2 NPs (SA NPs), and a mixture of CFR and SA NPs were synthesized in aqueous solution at low temperature. Furthermore, sea urchin-like nanostructures combining SA and CFR NPs, which were designated as anatase/rutile nanostructures (A/R NSs), were produced through the self-assembly of jack bean urease (JBU). The specific surface area of the A/R NSs was considerably larger than those of the CFR NPs, SA NPs, and commercial TiO 2 (P25). In addition, the amount of reactive oxygen species (ROS) yielded from the A/R NSs was significantly higher than that yielded from CFR NPs, SA NPs, and P25 because of the large surface area of the A/R NSs and a synergistic effect caused by the integration of anatase and rutile phases. The A/R NSs showed no cytotoxicity at concentrations <100 μg/mL, although CFR NPs, SA NPs, and P25 were cytotoxic, probably because of their size and shape. Using the high surface area and the superior photocatalytic activity of the A/R NSs, macrophages were effectively destroyed by UV irradiation for the purpose of treating atherosclerosis. Macrophages were killed more effectively by the A/R NSs than P25. Furthermore, different mechanisms of cell destruction resulting from UV irradiation, A/R NSs, and a combination of both were investigated. The death of cells treated with A/R NSs and exposed to UV irradiation was induced primarily by apoptosis rather than necrosis; cells that were not treated with the NSs died mainly from necrosis.

元の言語英語
ページ(範囲)3341-3347
ページ数7
ジャーナルChemistry of Materials
23
発行部数14
DOI
出版物ステータス出版済み - 7 26 2011
外部発表Yes

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Macrophages
Titanium dioxide
Self assembly
Nanostructures
Enzymes
Nanoparticles
titanium dioxide
Irradiation
Jacks
Cell death
Cytotoxicity
Specific surface area

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)
  • Materials Chemistry

これを引用

Formation of TiO2 nanostructures by enzyme-mediated self-assembly for the destruction of macrophages. / Hayashi, Koichiro; Nakamura, Michihiro; Sakamoto, Wataru; Yogo, Toshinobu; Kori, Toshinari; Ishimura, Kazunori.

:: Chemistry of Materials, 巻 23, 番号 14, 26.07.2011, p. 3341-3347.

研究成果: ジャーナルへの寄稿記事

Hayashi, Koichiro ; Nakamura, Michihiro ; Sakamoto, Wataru ; Yogo, Toshinobu ; Kori, Toshinari ; Ishimura, Kazunori. / Formation of TiO2 nanostructures by enzyme-mediated self-assembly for the destruction of macrophages. :: Chemistry of Materials. 2011 ; 巻 23, 番号 14. pp. 3341-3347.
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abstract = "Conifer foliage-like rutile TiO2 nanoparticles (CFR NPs), spherical anatase TiO2 NPs (SA NPs), and a mixture of CFR and SA NPs were synthesized in aqueous solution at low temperature. Furthermore, sea urchin-like nanostructures combining SA and CFR NPs, which were designated as anatase/rutile nanostructures (A/R NSs), were produced through the self-assembly of jack bean urease (JBU). The specific surface area of the A/R NSs was considerably larger than those of the CFR NPs, SA NPs, and commercial TiO 2 (P25). In addition, the amount of reactive oxygen species (ROS) yielded from the A/R NSs was significantly higher than that yielded from CFR NPs, SA NPs, and P25 because of the large surface area of the A/R NSs and a synergistic effect caused by the integration of anatase and rutile phases. The A/R NSs showed no cytotoxicity at concentrations <100 μg/mL, although CFR NPs, SA NPs, and P25 were cytotoxic, probably because of their size and shape. Using the high surface area and the superior photocatalytic activity of the A/R NSs, macrophages were effectively destroyed by UV irradiation for the purpose of treating atherosclerosis. Macrophages were killed more effectively by the A/R NSs than P25. Furthermore, different mechanisms of cell destruction resulting from UV irradiation, A/R NSs, and a combination of both were investigated. The death of cells treated with A/R NSs and exposed to UV irradiation was induced primarily by apoptosis rather than necrosis; cells that were not treated with the NSs died mainly from necrosis.",
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