Formulation of Nanoparticle-Eluting Stents by a Cationic Electrodeposition Coating Technology. Efficient Nano-Drug Delivery via Bioabsorbable Polymeric Nanoparticle-Eluting Stents in Porcine Coronary Arteries

Kaku Nakano, Kensuke Egashira, Seigo Masuda, Kouta Funakoshi, Gang Zhao, Satoshi Kimura, Tetsuya Matoba, Katsuo Sueishi, Yasuhisa Endo, Yoshiaki Kawashima, Kaori Hara, Hiroyuki Tsujimoto, Ryuji Tominaga, Kenji Sunagawa

研究成果: ジャーナルへの寄稿記事

93 引用 (Scopus)

抄録

Objectives: The objective of this study was to formulate a nanoparticle (NP)-eluting drug delivery stent system by a cationic electrodeposition coating technology. Background: Nanoparticle-mediated drug delivery systems (DDS) are poised to transform the development of innovative therapeutic devices. Therefore, we hypothesized that a bioabsorbable polymeric NP-eluting stent provides an efficient DDS that shows better and more prolonged delivery compared with dip-coating stent. Methods: We prepared cationic NP encapsulated with a fluorescence marker (FITC) by emulsion solvent diffusion method, succeeded to formulate an NP-eluting stent with a novel cation electrodeposition coating technology, and compared the in vitro and in vivo characteristics of the FITC-loaded NP-eluting stent with dip-coated FITC-eluting stent and bare metal stent. Results: The NP was taken up stably and efficiently by cultured vascular smooth muscle cells in vitro. In a porcine coronary artery model in vivo, substantial FITC fluorescence was observed in neointimal and medial layers of the stented segments that had received the FITC-NP-eluting stent until 4 weeks. In contrast, no substantial FITC fluorescence was observed in the segments from the polymer-based FITC-eluting stent or from bare metal stent. The magnitudes of stent-induced injury, inflammation, endothelial recovery, and neointima formation were comparable between bare metal stent and NP-eluting stent groups. Conclusions: Therefore, this NP-eluting stent is an efficient NP-mediated DDS that holds as an innovative platform for the delivery of less invasive nano-devices targeting cardiovascular disease.

元の言語英語
ページ(範囲)277-283
ページ数7
ジャーナルJACC: Cardiovascular Interventions
2
発行部数4
DOI
出版物ステータス出版済み - 4 1 2009

Fingerprint

Electroplating
Nanoparticles
Stents
Coronary Vessels
Swine
Technology
Fluorescein-5-isothiocyanate
Pharmaceutical Preparations
Drug Delivery Systems
Fluorescence
Metals
Metal Nanoparticles
Neointima
Equipment and Supplies
Emulsions
Vascular Smooth Muscle

All Science Journal Classification (ASJC) codes

  • Cardiology and Cardiovascular Medicine

これを引用

Formulation of Nanoparticle-Eluting Stents by a Cationic Electrodeposition Coating Technology. Efficient Nano-Drug Delivery via Bioabsorbable Polymeric Nanoparticle-Eluting Stents in Porcine Coronary Arteries. / Nakano, Kaku; Egashira, Kensuke; Masuda, Seigo; Funakoshi, Kouta; Zhao, Gang; Kimura, Satoshi; Matoba, Tetsuya; Sueishi, Katsuo; Endo, Yasuhisa; Kawashima, Yoshiaki; Hara, Kaori; Tsujimoto, Hiroyuki; Tominaga, Ryuji; Sunagawa, Kenji.

:: JACC: Cardiovascular Interventions, 巻 2, 番号 4, 01.04.2009, p. 277-283.

研究成果: ジャーナルへの寄稿記事

Nakano, Kaku ; Egashira, Kensuke ; Masuda, Seigo ; Funakoshi, Kouta ; Zhao, Gang ; Kimura, Satoshi ; Matoba, Tetsuya ; Sueishi, Katsuo ; Endo, Yasuhisa ; Kawashima, Yoshiaki ; Hara, Kaori ; Tsujimoto, Hiroyuki ; Tominaga, Ryuji ; Sunagawa, Kenji. / Formulation of Nanoparticle-Eluting Stents by a Cationic Electrodeposition Coating Technology. Efficient Nano-Drug Delivery via Bioabsorbable Polymeric Nanoparticle-Eluting Stents in Porcine Coronary Arteries. :: JACC: Cardiovascular Interventions. 2009 ; 巻 2, 番号 4. pp. 277-283.
@article{63e5178d04d346dd9e926210cbc8211f,
title = "Formulation of Nanoparticle-Eluting Stents by a Cationic Electrodeposition Coating Technology. Efficient Nano-Drug Delivery via Bioabsorbable Polymeric Nanoparticle-Eluting Stents in Porcine Coronary Arteries",
abstract = "Objectives: The objective of this study was to formulate a nanoparticle (NP)-eluting drug delivery stent system by a cationic electrodeposition coating technology. Background: Nanoparticle-mediated drug delivery systems (DDS) are poised to transform the development of innovative therapeutic devices. Therefore, we hypothesized that a bioabsorbable polymeric NP-eluting stent provides an efficient DDS that shows better and more prolonged delivery compared with dip-coating stent. Methods: We prepared cationic NP encapsulated with a fluorescence marker (FITC) by emulsion solvent diffusion method, succeeded to formulate an NP-eluting stent with a novel cation electrodeposition coating technology, and compared the in vitro and in vivo characteristics of the FITC-loaded NP-eluting stent with dip-coated FITC-eluting stent and bare metal stent. Results: The NP was taken up stably and efficiently by cultured vascular smooth muscle cells in vitro. In a porcine coronary artery model in vivo, substantial FITC fluorescence was observed in neointimal and medial layers of the stented segments that had received the FITC-NP-eluting stent until 4 weeks. In contrast, no substantial FITC fluorescence was observed in the segments from the polymer-based FITC-eluting stent or from bare metal stent. The magnitudes of stent-induced injury, inflammation, endothelial recovery, and neointima formation were comparable between bare metal stent and NP-eluting stent groups. Conclusions: Therefore, this NP-eluting stent is an efficient NP-mediated DDS that holds as an innovative platform for the delivery of less invasive nano-devices targeting cardiovascular disease.",
author = "Kaku Nakano and Kensuke Egashira and Seigo Masuda and Kouta Funakoshi and Gang Zhao and Satoshi Kimura and Tetsuya Matoba and Katsuo Sueishi and Yasuhisa Endo and Yoshiaki Kawashima and Kaori Hara and Hiroyuki Tsujimoto and Ryuji Tominaga and Kenji Sunagawa",
year = "2009",
month = "4",
day = "1",
doi = "10.1016/j.jcin.2008.08.023",
language = "English",
volume = "2",
pages = "277--283",
journal = "JACC: Cardiovascular Interventions",
issn = "1936-8798",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Formulation of Nanoparticle-Eluting Stents by a Cationic Electrodeposition Coating Technology. Efficient Nano-Drug Delivery via Bioabsorbable Polymeric Nanoparticle-Eluting Stents in Porcine Coronary Arteries

AU - Nakano, Kaku

AU - Egashira, Kensuke

AU - Masuda, Seigo

AU - Funakoshi, Kouta

AU - Zhao, Gang

AU - Kimura, Satoshi

AU - Matoba, Tetsuya

AU - Sueishi, Katsuo

AU - Endo, Yasuhisa

AU - Kawashima, Yoshiaki

AU - Hara, Kaori

AU - Tsujimoto, Hiroyuki

AU - Tominaga, Ryuji

AU - Sunagawa, Kenji

PY - 2009/4/1

Y1 - 2009/4/1

N2 - Objectives: The objective of this study was to formulate a nanoparticle (NP)-eluting drug delivery stent system by a cationic electrodeposition coating technology. Background: Nanoparticle-mediated drug delivery systems (DDS) are poised to transform the development of innovative therapeutic devices. Therefore, we hypothesized that a bioabsorbable polymeric NP-eluting stent provides an efficient DDS that shows better and more prolonged delivery compared with dip-coating stent. Methods: We prepared cationic NP encapsulated with a fluorescence marker (FITC) by emulsion solvent diffusion method, succeeded to formulate an NP-eluting stent with a novel cation electrodeposition coating technology, and compared the in vitro and in vivo characteristics of the FITC-loaded NP-eluting stent with dip-coated FITC-eluting stent and bare metal stent. Results: The NP was taken up stably and efficiently by cultured vascular smooth muscle cells in vitro. In a porcine coronary artery model in vivo, substantial FITC fluorescence was observed in neointimal and medial layers of the stented segments that had received the FITC-NP-eluting stent until 4 weeks. In contrast, no substantial FITC fluorescence was observed in the segments from the polymer-based FITC-eluting stent or from bare metal stent. The magnitudes of stent-induced injury, inflammation, endothelial recovery, and neointima formation were comparable between bare metal stent and NP-eluting stent groups. Conclusions: Therefore, this NP-eluting stent is an efficient NP-mediated DDS that holds as an innovative platform for the delivery of less invasive nano-devices targeting cardiovascular disease.

AB - Objectives: The objective of this study was to formulate a nanoparticle (NP)-eluting drug delivery stent system by a cationic electrodeposition coating technology. Background: Nanoparticle-mediated drug delivery systems (DDS) are poised to transform the development of innovative therapeutic devices. Therefore, we hypothesized that a bioabsorbable polymeric NP-eluting stent provides an efficient DDS that shows better and more prolonged delivery compared with dip-coating stent. Methods: We prepared cationic NP encapsulated with a fluorescence marker (FITC) by emulsion solvent diffusion method, succeeded to formulate an NP-eluting stent with a novel cation electrodeposition coating technology, and compared the in vitro and in vivo characteristics of the FITC-loaded NP-eluting stent with dip-coated FITC-eluting stent and bare metal stent. Results: The NP was taken up stably and efficiently by cultured vascular smooth muscle cells in vitro. In a porcine coronary artery model in vivo, substantial FITC fluorescence was observed in neointimal and medial layers of the stented segments that had received the FITC-NP-eluting stent until 4 weeks. In contrast, no substantial FITC fluorescence was observed in the segments from the polymer-based FITC-eluting stent or from bare metal stent. The magnitudes of stent-induced injury, inflammation, endothelial recovery, and neointima formation were comparable between bare metal stent and NP-eluting stent groups. Conclusions: Therefore, this NP-eluting stent is an efficient NP-mediated DDS that holds as an innovative platform for the delivery of less invasive nano-devices targeting cardiovascular disease.

UR - http://www.scopus.com/inward/record.url?scp=64749102952&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=64749102952&partnerID=8YFLogxK

U2 - 10.1016/j.jcin.2008.08.023

DO - 10.1016/j.jcin.2008.08.023

M3 - Article

VL - 2

SP - 277

EP - 283

JO - JACC: Cardiovascular Interventions

JF - JACC: Cardiovascular Interventions

SN - 1936-8798

IS - 4

ER -