FosB gene products trigger cell proliferation and morphological alteration with an increased expression of a novel processed form of galectin-1 in the rat 3Y1 embryo cell line

Tomoko Nishioka, Kunihiko Sakumi, Tomofumi Miura, Kazuki Tahara, Hidenori Horie, Toshihiko Kadoya, Yusaku Nakabeppu

研究成果: ジャーナルへの寄稿学術誌査読

23 被引用数 (Scopus)

抄録

In this study, we established rat 3Y1 embryo cell lines expressing FosB and ΔFosB as fusion proteins (ER-FosB, ER-ΔFosB) with the ligand-binding domain of human estrogen receptor (ER). The binding of estrogen to the fusion proteins resulted in their nuclear translocation. After estrogen administration, exponentially growing cells expressing ER- ΔFosB, and to a lesser extent ER-FosB, underwent morphological alteration from the flat fibroblastic shape to an extended bipolar shape, and ceased proliferating. Such morphological alteration was also induced in quiescent cells expressing ER-ΔFosB and ER-FosB after one round of cell division triggered by estrogen administration. The cells expressing ER-ΔFosB changed shape frequently, and the content of F-actin in the cytoplasm detected by binding of Alexa 488-phalloidin significantly decreased after the morphological alteration. By two-dimensional gel electrophoresis analysis of cellular proteins from the cells expressing ER-ΔFosB, we identified several proteins whose expression either increased or decreased after estrogen administration. Two of these proteins were identified from their amino acid sequences as novel processed form of galectin-1.

本文言語英語
ページ(範囲)653-661
ページ数9
ジャーナルJournal of biochemistry
131
5
DOI
出版ステータス出版済み - 5月 2002

!!!All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学

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