Four mood stabilizers commonly induce FEZ1 expression in human astrocytes

Zhiqian Yu, Chiaki Ono, Helen B. Kim, Hiroshi Komatsu, Yoichiro Tanabe, Nobutaka Sakae, Keiichi Nakayama, Hiroo Matsuoka, Ichiro Sora, William E. Bunney, Hiroaki Tomita

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

Objectives: Mood stabilizers influence the morphology, chemotaxis, and survival of neurons, which are considered to be related to the mood-stabilizing effects of these drugs. Although previous studies suggest glial abnormalities in patients with bipolar disorder and an effect of mood stabilizers on certain genes in astrocytes, less is known about the effects of mood stabilizers in astrocytes than in neurons. The present study identifies a common underlying response to mood stabilizers in astrocytes. Methods: Human astrocyte-derived cells (U-87 MG) were treated with the four most commonly used mood stabilizers (lithium, valproic acid, carbamazepine, and lamotrigine) and subjected to microarray gene expression analyses. The most prominently regulated genes were validated by qRT-PCR and western blot analysis. The intercellular localization of one of these regulated genes, fasciculation and elongation protein zeta 1 (FEZ1), was evaluated by immunofluorescence staining. Results: The microarray data indicated that FEZ1 was the only gene commonly induced by the four mood stabilizers in human astrocyte-derived cells. An independent experiment confirmed astrocytic FEZ1 induction at both the transcript and protein levels following mood stabilizer treatments. FEZ1 localized to the cytoplasm of transformed and primary astrocytes from the human adult brain. Conclusions: Our data suggest that FEZ1 may play important roles in human astrocytes, and that mood stabilizers might exert their cytoprotective and mood-stabilizing effects by inducing FEZ1 expression in astrocytes.

元の言語英語
ページ(範囲)486-499
ページ数14
ジャーナルBipolar Disorders
13
発行部数5-6
DOI
出版物ステータス出版済み - 8 1 2011

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Fasciculation
Astrocytes
Proteins
Genes
Neurons
Carbamazepine
Valproic Acid
Chemotaxis
Bipolar Disorder
Lithium
Neuroglia
Fluorescent Antibody Technique
Cytoplasm
Western Blotting
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Psychiatry and Mental health
  • Biological Psychiatry

これを引用

Yu, Z., Ono, C., Kim, H. B., Komatsu, H., Tanabe, Y., Sakae, N., ... Tomita, H. (2011). Four mood stabilizers commonly induce FEZ1 expression in human astrocytes. Bipolar Disorders, 13(5-6), 486-499. https://doi.org/10.1111/j.1399-5618.2011.00946.x

Four mood stabilizers commonly induce FEZ1 expression in human astrocytes. / Yu, Zhiqian; Ono, Chiaki; Kim, Helen B.; Komatsu, Hiroshi; Tanabe, Yoichiro; Sakae, Nobutaka; Nakayama, Keiichi; Matsuoka, Hiroo; Sora, Ichiro; Bunney, William E.; Tomita, Hiroaki.

:: Bipolar Disorders, 巻 13, 番号 5-6, 01.08.2011, p. 486-499.

研究成果: ジャーナルへの寄稿記事

Yu, Z, Ono, C, Kim, HB, Komatsu, H, Tanabe, Y, Sakae, N, Nakayama, K, Matsuoka, H, Sora, I, Bunney, WE & Tomita, H 2011, 'Four mood stabilizers commonly induce FEZ1 expression in human astrocytes', Bipolar Disorders, 巻. 13, 番号 5-6, pp. 486-499. https://doi.org/10.1111/j.1399-5618.2011.00946.x
Yu, Zhiqian ; Ono, Chiaki ; Kim, Helen B. ; Komatsu, Hiroshi ; Tanabe, Yoichiro ; Sakae, Nobutaka ; Nakayama, Keiichi ; Matsuoka, Hiroo ; Sora, Ichiro ; Bunney, William E. ; Tomita, Hiroaki. / Four mood stabilizers commonly induce FEZ1 expression in human astrocytes. :: Bipolar Disorders. 2011 ; 巻 13, 番号 5-6. pp. 486-499.
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abstract = "Objectives: Mood stabilizers influence the morphology, chemotaxis, and survival of neurons, which are considered to be related to the mood-stabilizing effects of these drugs. Although previous studies suggest glial abnormalities in patients with bipolar disorder and an effect of mood stabilizers on certain genes in astrocytes, less is known about the effects of mood stabilizers in astrocytes than in neurons. The present study identifies a common underlying response to mood stabilizers in astrocytes. Methods: Human astrocyte-derived cells (U-87 MG) were treated with the four most commonly used mood stabilizers (lithium, valproic acid, carbamazepine, and lamotrigine) and subjected to microarray gene expression analyses. The most prominently regulated genes were validated by qRT-PCR and western blot analysis. The intercellular localization of one of these regulated genes, fasciculation and elongation protein zeta 1 (FEZ1), was evaluated by immunofluorescence staining. Results: The microarray data indicated that FEZ1 was the only gene commonly induced by the four mood stabilizers in human astrocyte-derived cells. An independent experiment confirmed astrocytic FEZ1 induction at both the transcript and protein levels following mood stabilizer treatments. FEZ1 localized to the cytoplasm of transformed and primary astrocytes from the human adult brain. Conclusions: Our data suggest that FEZ1 may play important roles in human astrocytes, and that mood stabilizers might exert their cytoprotective and mood-stabilizing effects by inducing FEZ1 expression in astrocytes.",
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