Freeze stabilization and cryopreparation technique for visualizing the water distribution in woody tissues by X-ray imaging and cryo-scanning electron microscopy

Yasuhiro Utsumi, Yuzou Sano

研究成果: 著書/レポートタイプへの貢献

抄録

The protocol of freeze stabilization and cryopreparation techniques to examine the distribution of water in living tree stems by X-ray imaging and cryo-scanning electron microscopy have been developed and described. The brief procedures are as follows. Firstly, a portion of transpiring stem is frozen in the standing state with liquid nitrogen to stabilize the water that is present in the conducting tissue. After filling with liquid nitrogen, discs are then collected from the frozen portion of the stem and stored in liquid nitrogen. In a low-temperature room, the samples for X-ray imaging are sectioned with a fine handsaw, and the trimmed sample blokes for cryo-scanning electron microscopy are cleanly planed using a sliding microtome. Finally, the frozen sections are irradiated in a soft X-ray apparatus, and the small blocks are examined in cryo-scanning electron microscope after freeze-etching and metal coating.

元の言語英語
ホスト出版物のタイトルMethods in Molecular Biology
ページ677-688
ページ数12
1117
DOI
出版物ステータス出版済み - 2014

出版物シリーズ

名前Methods in Molecular Biology
1117
ISSN(印刷物)10643745

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Cryoelectron Microscopy
Nitrogen
X-Rays
Water
Freeze Etching
Frozen Sections
Metals
Electrons
Temperature

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

これを引用

Freeze stabilization and cryopreparation technique for visualizing the water distribution in woody tissues by X-ray imaging and cryo-scanning electron microscopy. / Utsumi, Yasuhiro; Sano, Yuzou.

Methods in Molecular Biology. 巻 1117 2014. p. 677-688 (Methods in Molecular Biology; 巻 1117).

研究成果: 著書/レポートタイプへの貢献

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