Freeze stabilization and cryopreparation technique for visualizing the water distribution in woody tissues by X-ray imaging and cryo-scanning electron microscopy

Yasuhiro Utsumi, Yuzou Sano

研究成果: Chapter in Book/Report/Conference proceedingChapter

4 被引用数 (Scopus)

抄録

The protocol of freeze stabilization and cryopreparation techniques to examine the distribution of water in living tree stems by X-ray imaging and cryo-scanning electron microscopy have been developed and described. The brief procedures are as follows. Firstly, a portion of transpiring stem is frozen in the standing state with liquid nitrogen to stabilize the water that is present in the conducting tissue. After filling with liquid nitrogen, discs are then collected from the frozen portion of the stem and stored in liquid nitrogen. In a low-temperature room, the samples for X-ray imaging are sectioned with a fine handsaw, and the trimmed sample blokes for cryo-scanning electron microscopy are cleanly planed using a sliding microtome. Finally, the frozen sections are irradiated in a soft X-ray apparatus, and the small blocks are examined in cryo-scanning electron microscope after freeze-etching and metal coating.

本文言語英語
ホスト出版物のタイトルElectron Microscopy
ホスト出版物のサブタイトルMethods and Protocols
出版社Humana Press Inc.
ページ677-688
ページ数12
ISBN(印刷版)9781627037754
DOI
出版ステータス出版済み - 2014

出版物シリーズ

名前Methods in Molecular Biology
1117
ISSN(印刷版)1064-3745

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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