Frequency of Foxp3+CD4+CD25+ T cells is associated with the phenotypes of allergic asthma

Koichiro Matsumoto, Hiromasa Inoue, Satoru Fukuyama, Keiko Kan-O, Miyuki Eguchi-Tsuda, Takafumi Matsumoto, Atsushi Moriwaki, Takako Nakano, Yoichi Nakanishi

研究成果: Contribution to journalArticle

28 被引用数 (Scopus)

抄録

Background and objective: A forkhead/winged-helix family transcriptional repressor, Foxp3, is highly expressed on CD4+CD25+ T regulatory cells. The role of Foxp3+CD4+CD25+ T regulatory cells in asthma remains to be elucidated. Using mouse models and peripheral blood mononuclear cells (PBMC) from subjects with allergic asthma, we aimed to explore whether Foxp3+CD4+CD25+ T regulatory cells associate with asthma phenotypes. Methods: Foxp3 +CD4+CD25+ T cells were detected by FACS and the correlation between the frequency of Foxp3+CD4 +CD25+ T cells and asthma phenotypes was assessed. Results: The frequency of Foxp3+CD4+CD25+ T cells among total CD4+CD25+ T cells in the lungs showed an inverse correlation with eosinophilic inflammation in BALB/c, A/J and C57BL/6 strains. In addition, the frequency of Foxp3+CD4+CD25 + T cells was inversely correlated with BHR and allergen-specific IgE levels in the serum of A/J mice. In BALB/c mice, the frequency of Foxp3 +CD4+CD25+ T cells correlated with the level of IL-10 in BAL fluid. The inverse correlation between the frequency of Foxp3 +CD4+CD25+ T cells and eosinophilic inflammation disappeared when mice were treated with anti-IL-10 receptor mAb during allergen challenge. Interestingly, intracellular cytokine staining of lung cells revealed that IL-10 was predominantly produced by Foxp3 -CD4+CD25+ T cells. The frequency of Foxp3 +CD4+CD25+ T cells among total CD4 +CD25+ T cells in PBMC of asthmatics was significantly lower than that of healthy subjects, although there was no significant correlation between the frequency of Foxp3+CD4+CD25 + T cells and asthma severity. Conclusions: These results suggest a role for lung Foxp3+CD4+CD25+ T cells in the regulation of asthma phenotypes, presumably through an IL-10-mediated mechanism.

本文言語英語
ページ(範囲)187-194
ページ数8
ジャーナルRespirology
14
2
DOI
出版ステータス出版済み - 3 2009

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

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