From Structure-Function Analyses to Protein Engineering for Practical Applications of DNA Ligase

Maiko Tanabe, Yoshizumi Ishino, Hirokazu Nishida

研究成果: Contribution to journalReview article査読

6 被引用数 (Scopus)

抄録

DNA ligases are indispensable in all living cells and ubiquitous in all organs. DNA ligases are broadly utilized in molecular biology research fields, such as genetic engineering and DNA sequencing technologies. Here we review the utilization of DNA ligases in a variety of in vitro gene manipulations, developed over the past several decades. During this period, fewer protein engineering attempts for DNA ligases have been made, as compared to those for DNA polymerases. We summarize the recent progress in the elucidation of the DNA ligation mechanisms obtained from the tertiary structures solved thus far, in each step of the ligation reaction scheme. We also present some examples of engineered DNA ligases, developed from the viewpoint of their three-dimensional structures.

本文言語英語
論文番号267570
ジャーナルArchaea
2015
DOI
出版ステータス出版済み - 2015

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Physiology
  • Ecology, Evolution, Behavior and Systematics

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