TY - JOUR
T1 - From structure to mechanism— understanding initiation of DNA replication
AU - Riera, Alberto
AU - Barbon, Marta
AU - Noguchi, Yasunori
AU - Reuter, L. Maximilian
AU - Schneider, Sarah
AU - Speck, Christian
N1 - Funding Information:
We thank Indiana Magdalou and Almut Caspary for critical reading of the manuscript. We thank the Biotechnology and Biological Sciences Research Council (BB/M003760/1 and BB/N000323/1), the Medical Research Council (MC_U120085811), and the Wellcome Trust (107903/Z/15/Z) for funding.
Publisher Copyright:
© 2017 Riera et al.
PY - 2017
Y1 - 2017
N2 - DNA replication results in the doubling of the genome prior to cell division. This process requires the assembly of 50 or more protein factors into a replication fork. Here, we review recent structural and biochemical insights that start to explain how specific proteins recognize DNA replication origins, load the replicative helicase on DNA, unwind DNA, synthesize new DNA strands, and reassemble chromatin. We focus on the minichromosome maintenance (MCM2–7) proteins, which form the core of the eukaryotic replication fork, as this complex undergoes major structural rearrangements in order to engage with DNA, regulate its DNA-unwinding activity, and maintain genome stability.
AB - DNA replication results in the doubling of the genome prior to cell division. This process requires the assembly of 50 or more protein factors into a replication fork. Here, we review recent structural and biochemical insights that start to explain how specific proteins recognize DNA replication origins, load the replicative helicase on DNA, unwind DNA, synthesize new DNA strands, and reassemble chromatin. We focus on the minichromosome maintenance (MCM2–7) proteins, which form the core of the eukaryotic replication fork, as this complex undergoes major structural rearrangements in order to engage with DNA, regulate its DNA-unwinding activity, and maintain genome stability.
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U2 - 10.1101/gad.298232.117
DO - 10.1101/gad.298232.117
M3 - Review article
C2 - 28717046
AN - SCOPUS:85025096530
SN - 0890-9369
VL - 31
SP - 1073
EP - 1088
JO - Genes and Development
JF - Genes and Development
IS - 11
ER -