Funcitonal interactions of an archael sliding clamp with mammalian clamp loader and DNA polymerase δ

研究成果: ジャーナルへの寄稿記事

22 引用 (Scopus)

抄録

Background: By the total genome sequencing of several archaeal organisms, it has been confirmed that many archaeal proteins related to genetic information systems, including DNA replication, transcription and translation, have similar sequences to those of eukaryotes. In eukaryotic DNA replication, proliferating cell nuclear antigen (PCNA) works in clamping DNA polymerases on the DNA template and accomplishes a processive DNA synthesis. Archaea encode PCNA homologues in their genomes and Pyrococcus furiosus PCNA (PfuPCNA) stimulates the DNA synthesizing activities of the DNA polymerases, Pol I and Pol II, in this organism. Results: We have demonstrated that PfuPCNA interacts functionally with calf thymus DNA polymerase δ (Pol δ) and stimulates its activity. Moreover, human replication factor C (RFC) enhances the PfuPCNA-dependent DNA synthesis activity of Pol δ, indicating that human RFC works as the clamp loader for PfuPCNA. These results showed that the three-dimensional structures of archaral PCNA and RFC are actually similar enough to their eukaryotic counterparts to allow a molecular substitution between the two biological domains, albeit at a lower efficiency. Conclusions: We found that the archaeal molecule interacts functionally with the eukaryotic members in the DNA replication process. This finding supports the idea that studies on the DNA replication mechanism of archaeal organisms will provide many important clues for understanding of the intricate molecular recognition that is inherent to the DNA replication machinery in Eukarya.

元の言語英語
ページ(範囲)699-706
ページ数8
ジャーナルGenes to Cells
6
発行部数8
DOI
出版物ステータス出版済み - 9 8 2001
外部発表Yes

Fingerprint

Proliferating Cell Nuclear Antigen
DNA-Directed DNA Polymerase
Pyrococcus furiosus
DNA Replication
Replication Protein C
DNA
Eukaryota
Archaeal Proteins
Genome
Archaea
Information Systems
Constriction

All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

これを引用

Funcitonal interactions of an archael sliding clamp with mammalian clamp loader and DNA polymerase δ. / Ishino, Yoshizumi; Tsurimoto, Toshiki; Ishino, Sonoko; Cann, Isaac K O.

:: Genes to Cells, 巻 6, 番号 8, 08.09.2001, p. 699-706.

研究成果: ジャーナルへの寄稿記事

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