Functional α and β T cell chain receptor messages can be detected in old but not in young athymic mice

Kenji Kishihara, Yasunobu Yoshikai, Goro Matsuzaki, Tak W. Mak, Kikuo Nomoto

    研究成果: Contribution to journalArticle査読

    39 被引用数 (Scopus)

    抄録

    The expression and sequences of T cell antigen receptor (TcR) α and β‐chain genes were investigated in the spleens from congenitally athymic (nude) mice. A small number of Thy‐1+ cells (∼7.0%) was found in nylon wool‐enriched spleen cells from young (8 weeks old) nude mice but no L3T4 or Lyt‐2 surface antigens were detected. These nude mice expressed only a low level of 1.0‐kb β‐chain mRNA and little or no α‐gene transcripts. On the other hand the number of Thy‐1+ spleen cells increased slightly (to 24%) in old (20 weeks old) nude mice and a small number of L3T4+ (10%) and/or Lyt‐2+ (5%) cells were detectable. “Full length” 1.7‐kb α‐chain and 1.3‐kb β‐chain messages could be found in nylon wool‐enriched spleen cells from old nude mice. Sequence analysis of the cDNA revealed that no functional α and β‐chain genes can be detected in the spleen cells of young athymic mice while some of the old mice with nu/nu genotype are composed of completely rearranged V‐(D)‐J‐C‐gene segments which encode potentially functional proteins. Interestingly, two of three independent cDNA clones encoding the α chain used the same Vα and Jα‐gene segments. These results suggest that extrathymic TcR α and β‐chain gene rearrangements do occur, though slowly, to some extent, in nude mice and may be responsible for the limited antigen and/or H‐2‐related T cell functions in these old athymic mice. The data further suggests that the TcR repertoire in athymic mice may also be limited.

    本文言語英語
    ページ(範囲)477-482
    ページ数6
    ジャーナルEuropean Journal of Immunology
    17
    4
    DOI
    出版ステータス出版済み - 1 1 1987

    All Science Journal Classification (ASJC) codes

    • 免疫アレルギー学
    • 免疫学

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