Functional analyses of the activation loop of phototropin2 in Arabidopsis

Shin ichiro Inoue, Tomonao Matsushita, Yuta Tomokiyo, Masaki Matsumoto, Keiichi I. Nakayama, Toshinori Kinoshita, Ken ichiro Shimazaki

研究成果: ジャーナルへの寄稿記事

50 引用 (Scopus)

抄録

Phototropins (phot1 and phot2) are autophosphorylating blue-light receptor kinases that mediate blue-light responses such as phototropism, chloroplast accumulation, and stomatal opening in Arabidopsis (Arabidopsis thaliana). Only phot2 induces the chloroplast avoidance response under strong blue light. The serine (Ser) residues of the kinase activation loop in phot1 are autophosphorylated by blue light, and autophosphorylation is essential for the phot1-mediated responses. However, the role of autophosphorylation in phot2 remains to be determined. In this study, we substituted the conserved residues of Ser-761 and Ser-763 with alanine (S761A S763A) in the phot2 activation loop and analyzed their function by investigating the phot2- mediated responses after the transformation of phot1 phot2 double mutant with this mutant phot2 gene. Transgenic plants expressing the mutant phot2 protein exhibited impaired responses in chloroplast movement, stomatal opening, phototropic bending, leaf flattening, and plant growth; and those expressing phot2 with S761D S763D mutations showed the normal responses. Substitution of both Ser-761 and Ser-763 with alanine in phot2 did not significantly affect the kinase activity in planta. From these results, we conclude that phosphorylation of Ser-761 and Ser-763 in the activation loop may be a common primary step for phot2-mediated responses.

元の言語英語
ページ(範囲)117-128
ページ数12
ジャーナルPlant physiology
156
発行部数1
DOI
出版物ステータス出版済み - 5 1 2011

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Activation Analysis
Arabidopsis
serine
Serine
blue light
Chloroplasts
Light
phosphotransferases (kinases)
chloroplasts
protein phosphorylation
Alanine
Phototropins
Phototropism
mutants
alanine
Phosphotransferases
Plant Leaves
stomatal movement
Protein-Serine-Threonine Kinases
phototropism

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science

これを引用

Functional analyses of the activation loop of phototropin2 in Arabidopsis. / Inoue, Shin ichiro; Matsushita, Tomonao; Tomokiyo, Yuta; Matsumoto, Masaki; Nakayama, Keiichi I.; Kinoshita, Toshinori; Shimazaki, Ken ichiro.

:: Plant physiology, 巻 156, 番号 1, 01.05.2011, p. 117-128.

研究成果: ジャーナルへの寄稿記事

Inoue, Shin ichiro ; Matsushita, Tomonao ; Tomokiyo, Yuta ; Matsumoto, Masaki ; Nakayama, Keiichi I. ; Kinoshita, Toshinori ; Shimazaki, Ken ichiro. / Functional analyses of the activation loop of phototropin2 in Arabidopsis. :: Plant physiology. 2011 ; 巻 156, 番号 1. pp. 117-128.
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