TY - JOUR
T1 - Functional analysis of an epsilon-class glutathione s-transferase from nilaparvata lugens (Hemiptera
T2 - Delphacidae)
AU - Saruta, Fumiko
AU - Yamada, Naotaka
AU - Yamamoto, Kohji
N1 - Funding Information:
This work was supported by the Japan Society for the Promotion of Science KAKENHI (grant number JP16KK0172 and 17K19272) and by a Research Grant for Young Investigators of Faculty of Agriculture, Kyushu University. Declarations of interest: none
Publisher Copyright:
© The Author(s) 2019.
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Glutathione conjugation is a crucial step in xenobiotic detoxification. In the current study, we have functionally characterized an epsilon-class glutathione S-transferase (GST) from a brown planthopper Nilaparvata lugens (nlGSTE). The amino acid sequence of nlGSTE revealed approximately 36-44% identity with epsilon-class GSTs of other species. The recombinant nlGSTE was prepared in soluble form by bacterial expression and was purified to homogeneity. Mutation experiments revealed that the putative substrate-binding sites, including Phe107, Arg112, Phe118, and Phe119, were important for glutathione transferase activity. Furthermore, inhibition study displayed that nlGSTE activity was affected by insecticides, proposing that, in brown planthopper, nlGSTE could recognize insecticides as substrates.
AB - Glutathione conjugation is a crucial step in xenobiotic detoxification. In the current study, we have functionally characterized an epsilon-class glutathione S-transferase (GST) from a brown planthopper Nilaparvata lugens (nlGSTE). The amino acid sequence of nlGSTE revealed approximately 36-44% identity with epsilon-class GSTs of other species. The recombinant nlGSTE was prepared in soluble form by bacterial expression and was purified to homogeneity. Mutation experiments revealed that the putative substrate-binding sites, including Phe107, Arg112, Phe118, and Phe119, were important for glutathione transferase activity. Furthermore, inhibition study displayed that nlGSTE activity was affected by insecticides, proposing that, in brown planthopper, nlGSTE could recognize insecticides as substrates.
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U2 - 10.1093/jisesa/iez096
DO - 10.1093/jisesa/iez096
M3 - Article
C2 - 31606747
AN - SCOPUS:85073148656
VL - 19
JO - Journal of Insect Science
JF - Journal of Insect Science
SN - 1536-2442
IS - 5
M1 - iez096
ER -