Functional analysis of genes involved in the biosynthesis of enterocin NKR-5-3B, a novel circular bacteriocin

Rodney H. Perez, Naoki Ishibashi, Tomoko Inoue, Kohei Himeno, Yoshimitsu Masuda, Narukiko Sawa, Takeshi Zendo, Pongtep Wilaipun, Vichien Leelawatcharamas, Jiro Nakayama, Kenji Sonomoto

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

A putative biosynthetic gene cluster of the enterocin NKR-5-3B (Ent53B), a novel circular bacteriocin, was analyzed by sequencing the flanking regions around enkB, the Ent53B structural gene, using a fosmid library. A region approximately 9 kb in length was obtained, and the enkB1, enkB2, enkB3, and enkB4 genes, encoding putative biosynthetic proteins involved in the production, maturation, and secretion of Ent53B, were identified. We also determined the identity of proteins mediating self-immunity against the effects of Ent53B. Heterologous expression systems in various heterologous hosts, such as Enterococcus faecalis and Lactococcus lactis strains, were successfully established. The production and secretion of the mature Ent53B required the cooperative functions of five genes. Ent53B was produced only by those heterologous hosts that expressed protein products of the enkB, enkB1, enkB2, enkB3, and enkB4 genes. Moreover, self-immunity against the antimicrobial action of Ent53B was conferred by at least two independent mechanisms. Heterologous hosts harboring the intact enkB4 gene and/or a combination of intact enkB1 and enkB3 genes were immune to the inhibitory action of Ent53B.

元の言語英語
ページ(範囲)291-300
ページ数10
ジャーナルJournal of bacteriology
198
発行部数2
DOI
出版物ステータス出版済み - 1 1 2016

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Bacteriocins
Genes
Immunity
Lactococcus lactis
enterocin
Enterococcus faecalis
Multigene Family
Recombinant Proteins
Libraries
Proteins

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

これを引用

Functional analysis of genes involved in the biosynthesis of enterocin NKR-5-3B, a novel circular bacteriocin. / Perez, Rodney H.; Ishibashi, Naoki; Inoue, Tomoko; Himeno, Kohei; Masuda, Yoshimitsu; Sawa, Narukiko; Zendo, Takeshi; Wilaipun, Pongtep; Leelawatcharamas, Vichien; Nakayama, Jiro; Sonomoto, Kenji.

:: Journal of bacteriology, 巻 198, 番号 2, 01.01.2016, p. 291-300.

研究成果: ジャーナルへの寄稿記事

Perez, RH, Ishibashi, N, Inoue, T, Himeno, K, Masuda, Y, Sawa, N, Zendo, T, Wilaipun, P, Leelawatcharamas, V, Nakayama, J & Sonomoto, K 2016, 'Functional analysis of genes involved in the biosynthesis of enterocin NKR-5-3B, a novel circular bacteriocin', Journal of bacteriology, 巻. 198, 番号 2, pp. 291-300. https://doi.org/10.1128/JB.00692-15
Perez, Rodney H. ; Ishibashi, Naoki ; Inoue, Tomoko ; Himeno, Kohei ; Masuda, Yoshimitsu ; Sawa, Narukiko ; Zendo, Takeshi ; Wilaipun, Pongtep ; Leelawatcharamas, Vichien ; Nakayama, Jiro ; Sonomoto, Kenji. / Functional analysis of genes involved in the biosynthesis of enterocin NKR-5-3B, a novel circular bacteriocin. :: Journal of bacteriology. 2016 ; 巻 198, 番号 2. pp. 291-300.
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